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Key Documents

PC205L

Sigma-Aldrich

Anti-Calcitonin Gene Related Peptide (CGRP) Rabbit pAb

lyophilized, Calbiochem®

Sinónimos:

Anti-CGRP

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.43

origen biológico

rabbit

Nivel de calidad

forma del anticuerpo

serum

tipo de anticuerpo

primary antibodies

clon

polyclonal

formulario

lyophilized

contiene

≤0.1% sodium azide as preservative

reactividad de especies

rat, mouse

fabricante / nombre comercial

Calbiochem®

condiciones de almacenamiento

OK to freeze

isotipo

IgG

Condiciones de envío

ambient

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

mouse ... Calca(12310)

Descripción general

Anti-CGRP, rabbit polyclonal, recognizes the α-CGRP in rat amygdala and spinal cord. It is validated for immunofluorescence, & for use with frozen, paraffin, and free-floating sections.
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the α-CGRP protein.
Recognizes the α-calcitonin gene related peptide in rat amygdala and spinal cord.

Inmunógeno

rat α-calcitonin gene related peptide (α-CGRP)

Aplicación


Free Floating Sections (see application references)
Frozen Sections (1:2000-1:4000, ABC detection)
Immunofluorescence (1:100-1:200, see application references)
Paraffin Sections (see application references; heat pre-treatment required; see comments)

Advertencia

Toxicity: Standard Handling (A)

Reconstitución

Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Carefully reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Avoid freeze/thaw cycles of solutions.

Nota de análisis

Positive Control
Rat amygdala or spinal cord

Otras notas

Antibody specificity was examined in the rat amygdala and spinal cord. Staining is completely eliminated by pre-treatment of antibody with 10-5M rat α-CGRP and partially eliminated by preabsorption with rat β-CGRP. Preabsorption with calcitonin resulted in no loss of immunostaining. For staining paraffin sections, heat sections to 80°C in 40 mM sodium citrate buffer, pH 8.5. Antibody should be titrated for optimal results in individual systems.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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