MAC148

Anti-Cas9 Antibody, clone 8G4

• Sinónimos: CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9
• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: rat
• Nivel de calidad: 200
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 8G4, monoclonal
• mol peso: calculated mol wt 158.44 kDa; observed mol wt ~210 kDa
• purificado por: using protein G
• reactividad de especies: all
• envase: antibody small pack of 100
• técnicas: immunofluorescence: suitable; western blot: suitable
• isotipo: IgG2aλ
• secuencia del epítopo: Unknown
• Nº de acceso Protein ID: NP_269215
• Nº de acceso UniProt: Q99ZW2
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: Streptococcus pyogenes ... Cas9(69900935)

Descripción

Especificidad

Clone 8G4 is a rat monoclonal antibody that specifically detects Cas9.

Inmunógeno

His-tagged, full-length, recombinant Cas9 from Streptococcus pyogenes.

Aplicación

Quality Control Testing

Evaluated by Western Blotting in lysate from HeLa cells overexpressing Cas9.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Cas9 in lysate from HeLa cells overexpressing Cas9.

Tested Applications

Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected Cas9 in Cell line stably expressing Cas9-NLS (A) versus wild type parental cell line (B) (Courtesy of Germán Camargo Ortega (Institute of Stem Cell Research, Helmholtz Munich,. Germany).

Western Blotting Analysis: A representative lot detected Cas9 in Western Blotting application. (Camargo Ortega, G., et al. (2019). Nature. 567(7746):113-117).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

CRISPR-associated endonuclease Cas9/Csn1 (UniProt: Q99ZW2; also known as SpCas9, SpyCas9, Cas9) is encoded by the Cas9 gene (Gene ID: 69900935) in Streptococcus pyogenes. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements, and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. Cas9 has a bi-lobed architecture with a recognition lobe (residues 41-425) and a discontinuous nuclease lobe (NUC, residues 1-40 and 453-1053); the crRNA-target DNA lies in a channel between the 2 lobes. The NUC lobe has 2 endonuclease domains. The discontinuous RuvC-like domain in NUC cleaves the target DNA non-complementary to crRNA while the HNH nuclease domain cleaves the target DNA complementary to crRNA. Cas9 serves as a RNA-guided DNA endonuclease that cleaves double-stranded DNA targets with a protospacer adjacent motif (PAM) and complementarity to the guide RNA. (Ref.: Fonfara, I., et al. (2014). Nucleic Acids Res. 42(4); 2577-2590; Jinek, M., et al. (2012). Science. 337(6096); 816-821).

Forma física

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitución

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommended storage: +2°C to +8°C.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable