MABT522

Anti-alpha-Tubulin Antibody, clone RM113

clone RM113, from rabbit

• Sinónimos: Tubulin alpha-1B chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain, alpha-Tubulin
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: RM113, monoclonal
• reactividad de especies: human
• técnicas: immunocytochemistry: suitable; western blot: suitable
• isotipo: IgG
• Nº de acceso UniProt: P68363
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... TUBA1A(7846)

Descripción

Descripción general

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

Inmunógeno

A peptide corresponding to the C-terminus of α-Tubulin.

Aplicación

Immunocytochemistry Analysis (ICC): A 1:200 dilution from a representative lot detected alpha-Tubulin in A431 cells.

This Anti-alpha-Tubulin Antibody, clone RM113 is validated for use in Western Blotting, Immunocytochemistry for the detection of alpha-Tubulin.

Calidad

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected alpha-Tubulin in A431 cell lysate.

Descripción de destino

~53 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Forma física

Format: Purified

Otras notas

Concentration: Please refer to lot specific datasheet.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash