MABT376

Anti-Ero1 Antibody, clone 2G4/12

clone 2G4/12, from mouse

• Sinónimos: ERO1-like protein alpha, ERO1-L, ERO1-L-alpha, Endoplasmic oxidoreductin-1-like protein, Oxidoreductin-1-L-alpha
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 2G4/12, monoclonal
• reactividad de especies: mouse, rat, human
• técnicas: immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG2aκ
• Nº de acceso NCBI: NP_055399
• Nº de acceso UniProt: Q96HE7
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... ERO1L(30001)

Descripción

Descripción general

ERO1-like protein alpha (ERO1) is also called endoplasmic oxidoreductin-1-like protein and Oxidoreductin-1-L-alpha. ERO1 is an essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. ERO1 is stimulated by hypoxia, indicating that it is regulated by the HIF-pathway. ERO1 may be responsible for much of the reactive oxygen species (ROS) in the cell, and therefore is a source of oxidative stress. EROS1 is required for the folding of immunoglobulin proteins, responsible for the release of the unfolded cholera toxin, and plays an important role in ER stress-induced, CHOP-dependent apoptosis. ERO1 is widely expressed at low levels, with high levels in upper digestive tract.

Inmunógeno

GST-tagged recombinant protein corresponding to Human Ero1.

Aplicación

Anti-Ero1 Antibody, clone 2G4/12 is a highly specific mouse monoclonal antibody, that targets ERO1-like protein alpha & has been tested in western blotting, IP & Immunofluorescence.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blotting Analysis: 1 µg/mL from a representative lot detected Ero1 from 10 µg of the following cell lysates--A431, HEK293, HeLa, HepG2, human placenta tissue, HUVEC, jurkat, L6, NIH/3T3, PC12, PC3, and RAW264.7.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Ero1 in Ero1 transfected HeLa cell lysate (Prof. R. Sitia, San Rafaele Scientific Institute, Milano, Italy).

Immunofluorescence Analysis: A representative lot detected Ero1 in HeLa cell lysate (Prof. R. Sitia, San Rafaele Scientific Institute, Milano, Italy).

Calidad

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Ero1 in 10 µg of HEK293 cell lysate.

Descripción de destino

~56 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
HEK293 cell lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable