MABT118

Anti-Desmoglein-1 Antibody, clone 32-2B

clone 32-2B, from mouse

• Sinónimos: Desmoglein-1, Desmosomal glycoprotein 1, DG1, DGI, Pemphigus foliaceus antigen
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 32-2B, monoclonal
• reactividad de especies: human
• reactividad de especies (predicha por homología): bovine (immunogen homology)
• técnicas: immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• isotipo: IgG2aκ
• Nº de acceso NCBI: NP_776470
• Nº de acceso UniProt: Q03763
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... DSG1(1828)

Descripción

Descripción general

Desmoglein-1 (DSG1) is a transmembrane glycoprotein belonging to the family of cadherins. It is one of the main structural components of desmosomal junctions which are abundant in tissues undergoing frequent mechanical stress, such as the heart, epidermis, lymphatic endothelial cells, and meninges. The desmoglein-1 subtype is highly expressed in the epidermis. Like other cadherins, desmoglein-1 contains multiple calcium binding sites and a cell-adhesion recognition site which facilitates intercellular adhesions. Desmoglein-1 is regulated by calcium and PKCα signaling, and is also coupled to intermediate filaments of the cytoskeleton. Desmosomes play a critical role in cell and tissue stability, and may contribute to the progression of cancer.

Inmunógeno

Bovine Desmoglein-1 from nasal epidermis

Aplicación

Anti-Desmoglein-1, clone 32-2B detects levels of Desmoglein-1 proteins & has been published & validated for use in WB, IF, IHC & ICC.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Desmoglein-1 in human tonsil tissues.

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Desmoglein-1 in human tonsil tissues.

Western Blot Analysis: A representative lot from an independent laboratory detected Desmoglein-1 in bovine epidermal desmosomal cores and in human keratinocyte cell lysates. (Vilela, M. J., et al. (1995). J Cell Sci. 108(Pt 4):1743-1750.).

Immunofluorescent Analysis: A representative lot from an independent laboratory detected Desmoglein-1 in MDCK cells (Vilela, M. J., et al. (1995). J Cell Sci. 108(Pt 4):1743-1750.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Desmoglein-1 in human epidermal tissue (Maruani, A., et al. (2008). Am J Clin Pathol. 130(3):369-374.).

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Calidad

Evaluated by Western Blot in human tonsil tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Desmoglein-1 in 10 µg of human tonsil tissue lysate.

Descripción de destino

~150 kDa observed. This protein may be glycosolated. Uniprot describes a molecular weight at ~112 kDa; however, Desmoglein-1 has been observed at ~150 kDa (Vilela, M. J., et al. (1995). J Cell Sci. 108(Pt 4):1743-1750.).

Forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
Human tonsil tissue lysate.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable