MABT100

Anti-Girdin Antibody, CT, clone 10E6.1

clone 10E6.1, from mouse

• Sinónimos: Girdin, Akt phosphorylation enhancer, Coiled-coil domain-containing protein 88A, G alpha-interacting vesicle-associated protein, GIV, Girders of actin filament, Hook-related protein 1, HkRP1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 10E6.1, monoclonal
• reactividad de especies: human
• técnicas: immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_001129069
• Nº de acceso UniProt: Q3V6T2
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CCDC88A(55704)

Descripción

Descripción general

Girdin (Ccdc88a; APE, GIV, or HkRP1) is a ubiquitous protein belonging to the CCDC88 family. Girdin is an actin-binding protein which interacts with actin via its C-terminal domain, and may play a crucial role in cell motility and cytoskeleton remodeling. These effects may be regulated by a reciprocal relationship between Girdin and Akt. Akt phosphorylates Girdin, and Girdin binds to Akt and enhances phosphorylation of Akt, thereby promoting phosphorylation of Akt’s downstream targets such as GSK-3β and FOXO1A. Girdin/Akt signaling may play an important role in cancer by facilitating the proliferation of cancer cells.

Inmunógeno

Recombinant protein corresponding to human Girdin.

Aplicación

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Girdin in epithelial cells of human prostate tissue.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Girdin in HeLa cells.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Girdin in COS-7 cell lysate.

This Anti-Girdin, C-terminus Antibody, clone 10E6.1 is validated for use in Western Blotting, IHC(P), ICC, Immunoprecipitation for the detection of Girdin, C-terminus, clone 10E6.1.

Calidad

Evaluated by Western Blot in human brain tissue lysate.

Wesetern Blot Analysis: A 1:5000 dilution of this antibody detected Girdin in 10 µg of human brain tissue lysate.

Descripción de destino

~216 kDa observed

Forma física

Format: Purified

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable