MABS483

Anti-PL Scramblase 1 Antibody, clone 4D2

clone 4D2, from mouse

• Sinónimos: Phospholipid scramblase 1, PL scramblase 1, Ca(2+)-dependent phospholipid scramblase 1, Erythrocyte phospholipid scramblase, MmTRA1b, PL Scramblase 1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 4D2, monoclonal
• reactividad de especies: human
• técnicas: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_066928
• Nº de acceso UniProt: O15162
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PLSCR1(5359)

Descripción

Descripción general

Phospholipid scramblase 1 (UniProt O15162; also known as Ca(2+)-dependent phospholipid scramblase 1, Erythrocyte phospholipid scramblase, MmTRA1b, PL scramblase 1) is encoded by the PLSCR1 (also known as MMTRA1B) gene (Gene ID 5359) in human. Plasma membrane phospholipids are distributed asymmetrically between the inner and outer leaflets. Such asymmetrical distribution collapses in response to blood coagulation and apoptosis, resulting in phospholipid “scrambling” between the two leaflets of the plasma membrane. Flippases, floppases, and scramblases are three types enzymes known to mediate transbilayer lipid motion. Flippases and floppases function via an ATP-dependent mechanism, while scramblases mediate transbilayer movement in a non-selective and energy-independent manner. Originally identified in 1996 as a 37 kDa erythrocyte type II transmembrane protein that mediates calcium-dependent membrane phospholipids redistribution, PL Scramblase 1 is the protein product encoded by the founding member of the PLSCR family of genes (PLSCR1-5). All PLSCR family members, with the exception of PLSCR2, possess a proline-rich N-terminal region containing PxxP and PPxY domains, a cysteine-rich region, a conserved calcium-binding domain (EF-hand-like), and a putative transmembrane region enriched in hydrophobic amino acids. In addition, PLSCR1 contains a nuclear localization signal (NLS) and a DNA-binding domain that are essential for its nuclear localization and associated nuclear function. In addition to PLSCRs, TMEM16 and XKR family members have also been reported to mediate scramblase activity.

Inmunógeno

Recombinant protein corresponding to human PL Scramblase 1.

Aplicación

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

This Anti-PL Scramblase 1 Antibody, clone 4D2 is validated for use in Western Blotting, Immunoprecipitation, Immunocytochemistry for the detection of PL Scramblase 1 .

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected PL Scramblase 1 in 10 µg of human erythroleukemia K562 and Jurkat cell lysate.
Immunocytochemistry Analysis: A representative lot detected endogenous PLSCR1 in paraformaldehyde-fixed, saponin-permeabilized HT1080 cells (25 µg mAb/mL) by confocal fluorescence microscopy (Zhou, Q., et al, (2000) 95(8):2593-2599).
Immunocytochemistry Analysis: A representative lot detected endogenous PLSCR1 in formaldehyde-fixed, Triton X-100-permeabilized HT1080 cells (5 µg mAb/mL) by confocal fluorescence microscopy (Wiedmer, T., et al, (2003) Biochemistry. 42(5):1227-1233).
Immunoprecipitation Analysis: A representative lot immunoprecipitated exogenously expressed human PLSCR1 constructs from lysates (25 µg mAb/0.5 mL lysate) of transfected murine SVT2 cells (Wiedmer, T., et al, (2003) Biochemistry. 42(5):1227-1233).
Western Blotting Analysis: A representative lot detected human PLSCR1 in lysates from varous human cell lines (PMID 10753839, 12564925, 15308560, 16091359).

Calidad

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PL Scramblase 1 in 10 µg of HeLa cell lysate.

Descripción de destino

~35 kDa observed

Forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable