MABS456
Anti-PTP1B Antibody (Oxidized), clone scFvs 45
clone scFvs 45, from chicken
Sinónimos:
Tyrosine-protein phosphatase non-receptor type 1, Protein-tyrosine phosphatase 1B, PTP-1B
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
chicken
Nivel de calidad
forma del anticuerpo
purified antibody
tipo de anticuerpo
primary antibodies
clon
scFvs 45, monoclonal
reactividad de especies
human
técnicas
immunoprecipitation (IP): suitable
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... PTPN1(5770)
Descripción general
PTP1B is the prototypic member of the protein tyrosine phosphatase (PTP) family, which comprises at least 37 proteins. The family is characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. The PTPs have high substrate specificity for phosphotyrosyl proteins. At the primary sequence level, PTPs share little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. PTP1B is a negative regulator of insulin and leptin signal transduction and may be a potential therapeutic target for diabetes and obesity. Insulin also influences the expression of splice variants of PTP1B.
Especificidad
This antibody only reacts with the oxidized form.
Inmunógeno
Epitope: PTP1B-OX
Recombinant protein corresponding to the PTP1B-OX of human PTP1B (Oxidized).
Aplicación
Anti-PTP1B Antibody (Oxidized), clone scFvs 45 | MABS456 is an antibody against PTP1B Antibody for use in Immunoprecipitation, Inhibits Activity/Function.
Immunoprecipitation Analysis: This antibody immunoprecipitated PTP1B (Oxidized) recombinant (aa 1-321; WT and CASA mutant) (Haque, A., et al. (2011). Cell. 147:185-198).
Immunoprecipitation Analysis: This antibody immunoprecipitated PTP1B (Oxidized) recombinant (aa 1-321; WT and CASA mutant) (Haque, A., et al. (2012). Nature America. 7(12):2127-2143).
Inhibits Activity/Function Analysis: A representative lot detected PTP1B (Oxidized) in recombinant PTP1B (aa 1-321; WT and CASA mutant) and scFv45) (Haque, A., et al. (2011). Cell. 147:185-198).
Inhibits Activity/Function Analysis: A representative lot detected PTP1B (Oxidized) in recombinant PTP1B (aa 1-321; WT and CASA mutant)and scFv45) (Haque, A., et al. (2012). Nature America. 7(12):2127-2143).
Immunoprecipitation Analysis: This antibody immunoprecipitated PTP1B (Oxidized) recombinant (aa 1-321; WT and CASA mutant) (Haque, A., et al. (2012). Nature America. 7(12):2127-2143).
Inhibits Activity/Function Analysis: A representative lot detected PTP1B (Oxidized) in recombinant PTP1B (aa 1-321; WT and CASA mutant) and scFv45) (Haque, A., et al. (2011). Cell. 147:185-198).
Inhibits Activity/Function Analysis: A representative lot detected PTP1B (Oxidized) in recombinant PTP1B (aa 1-321; WT and CASA mutant)and scFv45) (Haque, A., et al. (2012). Nature America. 7(12):2127-2143).
Research Category
Signaling
Signaling
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
Calidad
Evaluated by Immunoprecipitation in recombinant PTP1B (aa 1-321; WT and CASA mutant) and scFv45 cell lysate.
Immunoprecipitation Analysis: 278 ng of this antibody detected PTP1B (Oxidized) in recombinant PTP1B (aa 1-321; WT and CASA mutant) and scFv45 cell lysate.
Immunoprecipitation Analysis: 278 ng of this antibody detected PTP1B (Oxidized) in recombinant PTP1B (aa 1-321; WT and CASA mutant) and scFv45 cell lysate.
Descripción de destino
~37 kDa observed. The calculated molecular weight is 50kDa however PTP1B has been observed at ~37 kDa since it is a truncated form (aa 1-321). Uncharacterized band(s) may appear in some lysates.
Ligadura / enlace
Replaces: 04-1140
Forma física
Format: Purified
Ni-NTA agarose
Purified chicken monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Almacenamiento y estabilidad
Stable for 1 year at 2-8°C from date of receipt.
Otras notas
Concentration: Please refer to lot specific datasheet.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Cell
Haque, A; Andersen, JN; Salmeen, A; Barford, D; Tonks, NK
Cell null
Aftabul Haque et al.
Nature protocols, 7(12), 2127-2143 (2012-11-17)
We describe a phage display approach that we have previously used to generate conformation-sensor antibodies that specifically recognize and stabilize the oxidized, inactive conformation of protein tyrosine phosphatase 1B (PTP1B). We use a solution-based panning and screening strategy conducted in
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