MABS2292

Anti-SMCHD1 Antibody, clone 2B8

• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: rat
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 2B8 (#8), monoclonal
• mol peso: calculated mol wt 225.65 kDa; observed mol wt ~225 kDa
• reactividad de especies: mouse
• reactividad de especies (predicha por homología): human
• envase: antibody small pack of 100 μL
• técnicas: immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG2aκ
• Nº de acceso UniProt: Q6P5D8
• Condiciones de envío: dry ice
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

Structural maintenance of chromosomes flexible hinge domain-containing protein 1 (UniProt: Q6P5D8; also known as SMC hinge domain-containing protein 1) is encoded by the Smchd1 (also known as Kiaa0650) gene (Gene ID: 74355) in murine species. SMCHD1 is a homodimeric, non-canonical member of the structural maintenance of chromosomes (SMC) protein family that plays a key role in epigenetic silencing by regulating chromatin architecture. It is shown to be essential for X chromosome inactivation. It is recruited to inactivated chromosome X by Xist RNA and acts by mediating the merge of chromatin compartments. It is reported to localize to the inactive X via the XistHnrnpK-polycomb repressive complex 1 pathway. In addition to its role in X inactivation, it also plays a role in the expression of several autosomal gene clusters that are subject to monoallelic expression. It promotes heterochromatin formation in both autosomes and chromosome X, probably by mediating the merge of chromatin compartments. SMCHD1 also plays a role in DNA repair. It localizes to sites of DNA double-strand breaks in response to DNA damage to promote the repair of DNA double-strand breaks. It acts by promoting non-homologous end joining and inhibiting homologous recombination repair. Heterozygous mutations in Smchd1 gene have been linked to facioscapulohumeral muscular dystrophy 2, an inherited muscular weakness syndrome. (Ref.: Jansz, N., et al. (2018). Cell Rep. 25(7); 1912-1923; Mould, AW., et al. (2013). Epigenetics Chromatin 6(1); 19; Blewitt, ME., et al. (2008). Nat. Genet. 40(5); 663-669).

Especificidad

Clone 2B8 is a rat monoclonal antibody that detects SMC hinge domain-containing protein 1 (SMCHD1). It targets an epitope within the ATPase activity domain in the N-terminal half.

Inmunógeno

Recombinant fragment corresponding to 592 amino acids from the ATPase activity domain from the N-terminal half of murine Structural maintenance of chromosomes flexible hinge domain-containing protein 1 (SMCHD1).

Aplicación

Quality Control Testing

Evaluated by Western Blotting in lysate from wild-type Mouse neuronal stem cells.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected Structural maintenance of chromosomes flexible hinge domain-containing protein 1 (SMCHD1) in lysate from wild-type Mouse neuronal stem cells, but not in Smchd1 null cells.

Tested Applications

Western Blotting Analysis: A representative lot detected SMCHD1 in Western Blotting applications (Jansz, N., et. al. (2018). Cell Rep. 25(7):1912-1923).

Immunofluorescence Analysis: A representative lot detected SMCHD1 in Immunofluorescence applications (Jansz, N., et. al. (2018). Cell Rep. 25(7):1912-1923).

Immunoprecipitation Analysis: A representative lot immunoprecipitated SMCHD1 in Immunoprecipitation applications (Jansz, N., et. al. (2018). Cell Rep. 25(7):1912-1923).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-SMCHD1, clone 2B8, Cat. No. MABS2292, is a rat monoclonal antibody that detects Structural maintenance of chromosomes flexible hinge domain-containing protein 1 (SMCHD1) and is tested in Immunofluorescence, Immunoprecipitation, and Western Blotting.

Forma física

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable