MABS2066

Anti-LLGL1 Antibody, clone mAb17-35

clone mAb17-35, from mouse

• Sinónimos: Lethal(2) giant larvae protein homolog 1, Mgl-1, Mlgl
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: mAb17-35, monoclonal
• reactividad de especies: mouse, human
• envase: antibody small pack of 25 μg
• técnicas: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG2bκ
• Nº de acceso NCBI: NP_001152877.1
• Nº de acceso UniProt: Q80Y17
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: mouse ... Llgl1(16897)

Descripción

Descripción general

Lethal(2) giant larvae protein homolog 1 (UniProt: Q80Y17; also known as LLGL, Mgl-1, Mlgl) is encoded by the Llgl1 (also known as Llglh) gene (Gene ID: 16897) in murine species. LLGL belongs to the WD repeat L(2)GL family. LLGL is a cortical cytoskeleton protein that is found in a complex involved in maintenance of cell polarity and epithelial integrity. It is involved in the regulation of mitotic spindle orientation, proliferation, differentiation, and tissue organization of neuroepithelial cells. It is generally enriched in developing axons. LLGL stability and function is achieve by its interaction with RanBPM protein, which primarily acts as a scaffolding protein. Its N-terminal region containing SPRY domain is shown to be responsible for binding to RanBPM and this binding functionally extends its half-life by preventing its turnover through ubiquitin-proteasomal pathway. RanBPM is shown enhance tumor suppressor activity of LLGL by inhibiting LLGL-mediated cell proliferation and cell migration. (Ref.: Suresh, B., et al. (2010). J. Biol. Chem. 285(46); 35340-35349).

Especificidad

Clone mAb17-35 specifically detects LLGL1 in mouse. It targets an epitope with in 153 amino acids from the C-terminal region.

Inmunógeno

Epitope: C-terminus

GST-tagged recombinant fragment corresponding to 153 amino acids from the C-terminal region of murine Lethal(2) giant larvae protein homolog 1 (LLGL).

Aplicación

Anti-LLGL1, clone mAb17-35, Cat. No. MABS2066, is a mouse monoclonal antibody that detects Lethal(2) giant larvae protein homolog 1 and has been tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Research Category
Cell Structure

Western Blotting Analysis: A representative lot detected LLGL1 in Western Blotting applications (Ivanov, A.I., et. al. (2010). Am J Pathol. 176(1):134-45).

Immunocytochemistry Analysis: A representative lot detected LLGL1 in Immunocytochemistry applications (Ivanov, A.I., et. al. (2010). Am J Pathol. 176(1):134-45).

Immunohistochemistry Analysis: A representative lot detected LLGL1 in Immunohistochemistry applications (Weimer, J.M., et. al. (2009). Development. 136(17):2965-75).

Calidad

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected LLGL1 in mouse brain tissue lysate.

Descripción de destino

~120 kDa observed; 112.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forma física

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.