MABS195

Anti-Pan-Ras Antibody, clone RAS 10

clone RAS 10, from mouse

• Sinónimos: GTPase Kras, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, GTPase Kras, N-terminally processed
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• clon: RAS 10, monoclonal
• application: ICC; WB
• reactividad de especies: human
• técnicas: immunocytochemistry: suitable; western blot: suitable
• citations: 16

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: RAS 10, monoclonal
• reactividad de especies: human
• técnicas: immunocytochemistry: suitable; western blot: suitable
• isotipo: IgG2aκ
• Nº de acceso NCBI: NP_004976
• Nº de acceso UniProt: P01116
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... KRAS(3845)

Descripción

Descripción general

The human ras gene family consists of three identified members H-, K- and N-ras encoding proteins of 188-189 amino acids and 21,000 (p21) daltons. Human H- and K-ras are the homologues of v-H- and v-K-ras, sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes are referred to as proto-oncogenes, and have the potential for activation to oncogenes by mutations occurring in codons 12, 13 and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of ras proto-oncogene proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21s and their relevance to human disease diagnosis and prognosis is still emerging.

Inmunógeno

Recombinant protein corresponding to human Pan-Ras.

Aplicación

Detect Pan-Ras using this Anti-Pan-Ras Antibody, clone RAS 10 validated for use in Western Blotting, ICC.

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

Western Blot Analysis: A representative lot of this antibody detected Pan-Ras in SW-480 cell lysate.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Pan-Ras in A431 cells.

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Pan-Ras in IP (Hamer, P.J., et al. (1990). Hybridoma. 9(6):573-587.).

Calidad

Evaluated by Western Blot in MCF7 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Pan-Ras in 10 µg of MCF7 cell lysate.

Descripción de destino

~21 kDa observed

Forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
MCF7 cell lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable