MABS1914

Anti-Mitochondrial Pyruvate Carrier 2 (BRP44)

clone n/a, from mouse

• Sinónimos: Mitochondrial pyruvate carrier 2, Brain protein 44, BRP44
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: n/a, monoclonal
• reactividad de especies: mouse, human
• reactividad de especies (predicha por homología): rat (based on 100% sequence homology)
• envase: antibody small pack of 25 μg
• técnicas: immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_001137146
• Nº de acceso UniProt: O95563
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... MPC2(25874)

Descripción

Descripción general

Mitochondrial pyruvate carrier 2 (UniProt: O95563; also known as Brain protein 44, MPC2) is encoded by the MPC2 (also known as BRP44) in human. MPC2 is a multi-pass inner mitochondrial membrane protein that mediates the imports pyruvate into mitochondria. Pyruvate is an essential component in intermediary metabolism that occupies a key crossroads position between cytosolic glycolysis and mitochondrial metabolism. MPC2 contains 3 helical domains (aa 41-61; 73-90; and 96-115). In mammalian cells mitochondrial pyruvate carrier is composed on two paralogous subunits known as MPC1 and MPC2 that heteromerize to form a functional complex. It has been reported that MPC2 can also homomerize, however only MPC1/MPC2 heteromeric forms are functional. Complete absence of MPC2 is shown to cause embryonic lethality in murine models, however, murine cell lines that express an N-terminal truncated MPC2 protein (Mpc2 16) are reported to be viable, but exhibit significantly reduced capacity for mitochondrial pyruvate oxidation. (Ref.: Compan, V., et al. (2015). Mol. Cell 59(3); 491-501; Vigueira, PA., et al. (2014). Cell Rep. 7(6); 2042-2053).

Especificidad

This mouse monoclonal antibody detects Mitochondrial Pyruvate carrrier Protein 2 in human and mouse cells. It targets an epitope within 10 amino acids from the internal region.

Inmunógeno

A linear peptide corresponding to 10 amino acids from the internal region of human mitochondrial pyruvate carrier 2 protein.

Aplicación

Anti-Mitochondrial Pyruvate Carrier 2 (BRP44), Cat. No. MABS1914, is a mouse monoclonal antibody that detects human and mouse Mitochondrial pyruvate carrier 2 and has been tested for use in Immunoprecipitation and Western Blotting.

Research Category
Signaling

Western Blotting Analysis: 1 µg/mL from a representative lot detected Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse heart and mouse liver tissue lysates.

Immunoprecipitation Analysis: A 1:1,000 dilution from a representative lot immunoprecipitated Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse liver mitoplasts (Courtesy of Prof. Jean-Claude Martinou, Ph.D., University of Geneva, Switzerland)..

Western Blotting Analysis: 4 µg/mL from a represenetative lot detected Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse pancreas tissue lysate.

Western Blotting Analysis: A representative lot detected Mitochondrial Pyruvate Carrier 2 (BRP44) in Western Blotting applications (Compan, V., et. al. (2015). Mol Cell. 59(3):491-501; Vanderperre, B., et. al. (2016). PLoS Genet. 12(5):e1006056).

Calidad

Evaluated by Western Blotting in mouse liver mitochondrial lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Mitochondrial Pyruvate Carrier 2 (BRP44) in mouse liver mitochondrial lysate.

Descripción de destino

~15 kDa observed; 14.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forma física

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash