MABS1890

Anti-BMPR-II Antibody, clone 9F4.1

clone 9F4.1, from mouse

• Sinónimos: Bone morphogenetic protein receptor type-2, EC: 2.7.11.30, BMP type-2 receptor, BMPR-2, Bone morphogenetic protein receptor type II, BMP type II receptor
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 9F4.1, monoclonal
• reactividad de especies: human
• técnicas: immunohistochemistry: suitable (paraffin); western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_001195
• Nº de acceso UniProt: Q13873
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... BMPR2(659)

Descripción

Descripción general

Bone morphogenetic protein receptor type-2 (UniProt: Q13873; also known as EX: 2.7.11.30, BMP type-2 receptor, BMPR-2, Bone morphogenetic protein receptor type II, BMP type II receptor, BMPR-II) is encoded by the BMPR2 (also known as PPH1) gene (Gene ID: 659) in human. BMPR-2 is a single-pass type I membrane protein that is highly expressed in heart and liver. It binds Bone morphogenetic proteins that are involved in paracrine signaling. Two isoforms of BMPR-2 have been described that are produced by alternative splicing. BMPR-2 serves as a serine/threonine receptor kinase and upon ligand binding it forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors, which autophosphorylate, then bind and activate SMAD transcriptional regulators. It is shown to bind to BMP7, BMP2 and, less efficiently, BMP4. BMPR-2 is synthesized with a signal peptide (aa 1-16), which is subsequently cleaved off. The mature form has an extracellular domain (aa 27-150), a transmembrane domain (aa 151-171), and a cytoplasmic domain (aa 172-1038). Its kinase activity is localized within amino acids 203-504 in the cytoplasmic domain. Mutations in BMPR2 gene are reported to cause primary pulmonary hypertension that is characterized by plexiform lesions of proliferating endothelial cells in pulmonary arterioles. The lesions lead to elevated pulmonary arterial pression, right ventricular failure, and death.

Especificidad

Clone 9F4.1 detects BMP type-2 receptor in human cells. It targets an epitope within 111 amino acids from the C-terminal half.

Inmunógeno

GST/His-tagged recombinant fragment corresponding to 111 amino acids from the C-terminal half of human BMP type-2 receptor.

Aplicación

Anti-BMPR-II, clone 9F4.1, Cat. No. MABS1890, is a mouse monoclonal antibody that detects Bone morphogenetic protein receptor type-2 and has been tested for use in Western Blotting and Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected BMPR-II in human lymphoma, human pancreas, and human cervical cancer tissues.

Research Category
Signaling

Calidad

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected BMPR-II in 10 µg of HepG2 cell lysate.

Descripción de destino

~115 kDa observed, 115.20 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forma física

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash