MABS1704

Anti-Potassium Channel Kir6.2 Antibody, clone 10C1.1

clone 10C1.1, from mouse

• Sinónimos: ATP-sensitive inward rectifier potassium channel 11, IKATP, Inward rectifier K(+) channel Kir6.2, Potassium channel, inwardly rectifying subfamily J member 11
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 10C1.1, monoclonal
• reactividad de especies: human, rat
• técnicas: western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_001159762
• Nº de acceso UniProt: Q14654
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... KCNJ11(3767)

Descripción

Descripción general

ATP-sensitive inward rectifier potassium channel 11 (UniProt Q14654; also known as IKATP, Inward rectifier K(+) channel Kir6.2, Potassium channel, inwardly rectifying subfamily J member 11) is encoded by the KCNJ11 (also known as BIR, HHF2, MODY13, PNDM, TNDM3) gene (Gene ID 3767) in human. Inward rectifier K+ channels (Kirs) modulate cell membrane potential and are classified as K+ transport channels (Kir1.x, Kir4.x, Kir5.x, Kir7.x), classic Kir (Kir2.x), G-protein-gated Kirs (Kir2.x and Kir3.x), and ATP-sensitive Kirs (Kir6.x). Kirs mediate diverse physiological functions of both excitable and nonexcitable epithelial cells, such as cell adhesion-migration, volume regulation, apoptosis, and proliferation. ATP-sensitive potassium (K-ATP) channels are hetero-octamers composed of pore-forming Kir6.x (6.1 or 6.2) subunits and sulfonylurea receptor (SUR1 or SUR2) regulatory subunits. K-ATP channels are regulated by intracellular ATP and ADP concentrations and are highly expressed in cardiomyocytes, where they play crucial roles in ischemic preconditioning (IPC). Potassium channel openers mimic the cardioprotection, while K-ATP channel blocker or Kir6.2-knockout is shown to abolish the effect of IPC in mice. Human Kir6.2 is a 390-amino acid multi-pass membrane (a.a. 69-93, 117-128, 145-166) protein that contains two small extraceullar regions (a.a. 94-116 and 136-144) and a pore-forming domain (a.a. 117-128), having both its N- and C-terminal exposed intracellularly (a.a. 1-68 and a.a. 167-390).

Especificidad

Clone 10C1.1 targets human Kir6.2 N-terminal cytoplasmic region not present in the alternatively spliced isoform 2.

Inmunógeno

Epitope: N-terminal cytoplasmic domain.

KLH-conjugated linear peptide corresponding an N-terminal cytoplasmic domain sequence of human potassium channel Kir6.2.

Aplicación

Research Category
Signaling

Research Sub Category
Ion Channels & Transporters

This Anti-Potassium Channel Kir6.2 Antibody, clone 10C1.1 is validated for use in Western Blotting for the detection of Potassium Channel Kir6.2.

Western Blotting Analysis: A 1:1000 dilution from a representative lot detected potassium channel Kir6.2 in 10 µg of rat pancreas tissue lysate.

Calidad

Evaluated by Western Blotting in human pancreas tissue lysate.

Western Blotting Analysis: A 1:1000 dilution of this antibody detected potassium channel Kir6.2 in 10 µg of human pancreas tissue lysate.

Descripción de destino

~45 kDa observed. 43.54 kDa (human) and 43.61 kDa (rat) calculated.

Forma física

Format: Purified

Protein G purified.

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable