MABS1245

Anti-Thrombomodulin Antibody, clone 273-34A

clone 273-34A, from rat

• Sinónimos: TM, Thrombomodulin, Fetomodulin, CD141
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rat
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 273-34A, monoclonal
• reactividad de especies: mouse, human
• técnicas: ELISA: suitable; electron microscopy: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable
• isotipo: IgG2aκ
• Nº de acceso NCBI: NP_033404.1
• Nº de acceso UniProt: P15306
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... THBD(7056) ; mouse ... Thbd(21824)

Descripción

Descripción general

Thrombomodulin (UniProt P15306; also known as CD141, Fetomodulin, SnoRNA MBII-339, TM) is encoded by the Thbd gene (Gene ID 21824) in murine species. Thrombomodulin (TM) is an endothelial cell surface protein that function to suppress the propagation of thrombin (activated blood-coagulation factor II) generation by acting as a thrombin receptor. Formation of the TM-thrombin complex abolishes thrombin′s procoagulant activity by altering its substrate specificity, leading to proteolytic cleavage of protein C into the natural anticoagulant termed "activated protein C". Together with protein S, activated protein C proteolytically cleaves activated coagulation factors Va and VIIIa, thereby preventing the generation of activated coagulation factors Xa and thrombin. Murine thrombomodulin is initially produced with an N-terminal propeptide (a.a. 1-16) sequence, the removal of which yields the mature protein with a large extracellular region (a.a. 17-517), a transmembrane segment (a.a. 518-541), and a short cytoplamic tail (a.a. 542-577).

Inmunógeno

Epitope: Extracellular domain

Normal mouse lung microsomal fraction.

Aplicación

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Thrombomodulin in human kidney tissue.
Immunoprecipitation Analysis: Representative lots immunoprecipitated the 112 kDa thrombomodulin from normal mouse lung fibroblasts (NMLF), but not Line 1 mouse lung carcinoma cells (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701; Kennel, S.J., et al. (1987). Exp. Mol. Pathol. 47(1):110-124).
Immunohistochemistry Analysis: A representative lot detected upregulated thrombomodulin immunoreactivity associated with the neoepidermis during wound healing using formalin-fixed, paraffin-embedded mouse skin sections (Peterson, J.J., et al. (1999). Am. J. Pathol. 155(5): 1569–1575).
Immunohistochemistry Analysis: Representative lots detected epithelium thrombomodulin immunoreactivity in paraffin-embedded mouse lung sections (Ford, V.A., et al. (1992). J. Biol. Chem. 267(8):5446-5450; Kennel, S.J., et al. (1987). Exp. Mol. Pathol. 47(1):110-124).
Immunohistochemistry Analysis: A representative lot detected thrombomodulin immunoreactivity associated with capillary endothelial cells by fluorescent immunohistochemistry using frozen mouse lung sections (Kennel, S.J., et al. (1987). Exp. Mol. Pathol. 47(1):110-124)
ELISA Analysis: Representative lots were used as the capture antibody for the detection of thrombomodulin in mouse tissue samples by sandwich ELISA using clone 411-201B (Cat. No. MABS1273) as the detection antibody, followed by an HRP-conjugated secondary antibody, or by sandwich radio-immunoassay using 125I-labeled clone 411-201B as the detection antibody (Isermann, B., et al. (2001). Development. 128(6):827-838; Conway, E.M., et al. (1999). Blood. 93(10):3442-3450; Weiler-Guettler, H., et al. (1996). Circ. Res. 78(2):180-187; Ford, V.A., et al. (1992). J. Biol. Chem. 267(8):5446-5450).
Electron Microscopy Analysis: Representative lots detected thrombomodulin immunoreactivity associated with capillary endothelial cells by EM using frozen mouse lung sections (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701; Kennel, S.J., et al. (1987). Exp. Mol. Pathol. 47(1):110-124).
Western Blotting Analysis: A representative lot detected purified thrombomodulin (P112) from normal mouse lung fibroblasts (NMLF) (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701).

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

This Anti-Thrombomodulin Antibody, clone 273-34A is validated for use in Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Paraffin), ELISA, Electron Microscopy for the detection of Thrombomodulin.

Calidad

Evaluated by Immunohistochemistry in mouse heart tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Thrombomodulin in mouse heart tissue.

Descripción de destino

~ 62 kDa calculated

Forma física

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable