MABN827

Anti-Tau Antibody, clone T49 (Not human)

clone T49, from mouse

• Sinónimos: Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• clon: T49, monoclonal
• application: IHC; WB
• reactividad de especies: mouse, rat, bovine
• técnicas: immunohistochemistry: suitable; western blot: suitable
• citations: 14

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: T49, monoclonal
• reactividad de especies: mouse, rat, bovine
• no debe reaccionar con: human
• técnicas: immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_776531
• Nº de acceso UniProt: P29172
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... MAPT(4137) ; mouse ... Mapt(17762) , Mapt(281296) ; rat ... Mapt(29477)

Descripción

Descripción general

Tau or Microtubule-associated protein tau (MAPT), also known as neurofibrillary tangle protein and paired helical filament-tau (PHF-tau), is a cytosolic protein that promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of axonal polarity in neurons. Tau binds to and is thought to function as a linker protein between axonal microtubules and neural plasma membrane components. There are multiple isoforms, and the short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. PAD is the phosphatase activating domain, and has been demonstrated to be involved in the inhibition of anterograde, kinesin-based fast axonal transport (FAT) by activating axonal protein phosphatase 1 (PP1) and glycogen synthase kinase 3 (GSK3), independent of microtubule binding. Defects in Tau are thought to be the cause of a number of neurodegenerative diseases, including frontotemporal dementia (FTD), pallido-ponto-nigral degeneration (PPND), Pick disease of the brain (PIDB), corticobasal degeneration (CBD), supranuclear palsy type 1 (PSNP1), Alzheimer disease, and Parkinson disease. Clone T49 exhibits immunoreactivity against bovine, rat, and murine, but not human, Tau (UniProt P29172, P19332, P10637, P10636, respectively).

Inmunógeno

Purified corresponding to bovine Tau.

Aplicación

Anti-Tau Antibody, clone T49 (Not human) is an antibody against Tau for use in Western Blotting and Immunohistochemistry.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Tau in mouse cerebral cortex, mouse kidney, and mouse small intestine tissue.
Western Blotting Analysis: A representative lot detected Tau in PS19 neurons treated with PBS or transduced with strain A or strain B FL a-syn pffs (Guo, J.L., et al. (2013). Cell. 154:103-117).

Calidad

Evaluated by Western Blotting in mouse and human brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Tau in 10 µg of mouse and human brain tissue lysate.

Descripción de destino

~53 kDa observed. This protein has multiple isoforms which range from 45-76 kDa

Forma física

Format: Purified

Otras notas

Concentration: Please refer to lot specific datasheet.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable