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Key Documents

MABN486

Sigma-Aldrich

Anti-Co-Rest/RCOR1 Antibody, clone K72/8

clone K72/8, from mouse

Sinónimos:

REST corepressor 1, Protein CoREST

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

K72/8, monoclonal

reactividad de especies

rat, human, mouse

técnicas

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotipo

IgG1

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... RCOR1(23186)

Descripción general

REST corepressor 1(RCOR1, CoREST) is an essential component of the BHC corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. RCOR1 also promotes the demethylase activity of KDM1A on core histones and nucleosomal substrates and plays a central role in demethylation of Lys-4 of histone H3. Furthermore, RCOR1 is a component of a RCOR/GFI/KDM1A/HDAC complex that suppresses a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. RCOR1 is ubiquitously expressed and is primarily found in the nucleus, but is partially translocated into the cytoplasm upon HSV-1 infection.

Especificidad

This antibody may cross react with human RCOR3 based upon 70% sequence homology.

Inmunógeno

Recombinant protein corresponding to human Co-Rest/RCOR1.

Aplicación

Anti-Co-Rest/RCOR1 Antibody, clone K72/8 is a highly specific mouse monoclonal antibody, that targets CoRest & has been tested in western blotting, IHC & ICC.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Co-Rest/RCOR1 in human stomach tissue.
Immunocytochemistry Analysis: A representative lot detected Co-Rest/RCOR1 in COS-1 cells expressing Myc-tagged Co-Rest. (Prof. J. Trimmer, University of California, Davis.).
Research Category
Neuroscience
Research Sub Category
Developmental Signaling

Calidad

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Co-Rest/RCOR1 in 10 µg of Jurkat cell lysate.

Descripción de destino

~ 65 kDa observed. The calculated molecular weight is 53 kDa, however Co-Rest/RCOR1 has been shown as a ~65-70 kDa band in western blots (You, A., et al. (2001). National Academy of Sciences. 98(4):1454-1458)(Min, G. L., et al. (2005). Nature. 437:432-435).

Forma física

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
Jurkat cell lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Yan Xiong et al.
The Journal of clinical investigation, 130(4), 1830-1842 (2020-01-10)
Foxp3+ Tregs are key to immune homeostasis, but the contributions of various large, multiprotein complexes that regulate gene expression remain unexplored. We analyzed the role in Tregs of the evolutionarily conserved CoREST complex, consisting of a scaffolding protein, Rcor1 or
Seda Kilinc et al.
Molecular and cellular neurosciences, 74, 58-70 (2016-03-08)
Mammalian olfaction depends on the development of specialized olfactory sensory neurons (OSNs) that each express one odorant receptor (OR) protein from a large family of OR genes encoded in the genome. The lysine-specific demethylase-1 (LSD1) protein removes activating H3K4 or

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