MABN483

Anti-IRAP Antibody, Clone 3E1

clone 3E1, from mouse

• Sinónimos: Leucyl-cystinyl aminopeptidase, Cystinyl aminopeptidase, Insulin-regulated membrane aminopeptidase, Insulin-responsive aminopeptidase, IRAP, Oxytocinase, OTase, Placental leucine aminopeptidase, P-LAP, Leucyl-cystinyl aminopeptidase, pregnancy serum form
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• clon: 3E1, monoclonal
• application: ICC; WB
• reactividad de especies: human, mouse
• técnicas: immunocytochemistry: suitable; western blot: suitable
• citations: 1

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 3E1, monoclonal
• reactividad de especies: human, mouse
• técnicas: immunocytochemistry: suitable; western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_005566
• Nº de acceso UniProt: Q9UIQ6
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... LNPEP(4012)

Descripción

Descripción general

IRAP, also known as Leucyl-cystinyl aminopeptidase, Cystinyl aminopeptidase, Insulin-regulated membrane aminopeptidase, Insulin-responsive aminopeptidase (IRAP), Oxytocinase (OTase), or Placental leucine aminopeptidase (P-LAP) and encoded by the human gene name IRAP/OTASE, is an important aminopeptidase that plays an important role in maintaining homeostasis during pregnancy. IRAP degrades peptide hormones including oxytocin, vasopressin, and angiotensin III; it also appears to play a role in degrading neuronal peptides in the brain such as Met-enkephalin and dynorphin. IRAP also binds angiotensin IV and appears to be the receptor for angiotensin IV in the brain. IRAP is a membrane bound aminopeptidase and is often found within membrane vesicles that together with other proteins like GLUT4 translocates to the plasma membrane in response to insulin or oxytocin. A cleaved fragment of IRAP, the Leucyl-cystinyl aminopeptidase, is found in the blood during pregnancy, low in the first trimester but rising rapidly until birth, whereby it decreases rapidly, thus the secreted form is often used as a pregnancy marker. IRAP is highly expressed in placenta, heart, kidney and small intestine. IRAP is detected at lower levels in neuronal cells in the brain, in skeletal muscle, spleen, liver, testes and colon.

Inmunógeno

Epitope: N-terminus

Recombinant protein corresponding to the N-terminus of human IRAP.

Aplicación

Anti-IRAP, Clone 3E1 is an antibody against IRAP for use in WB, ICC.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Western Blotting Analysis: A representative lot detected IRAP in 3T3-L1 cells treated with or without insulin (Garza, L.A., et al. (2000). JBC. 275(4):2560-2567).
Immunocytochemistry Analysis: A representative lot detected IRAP in 3T3-L1 cells treated with or without insulin (Garza, L.A., et al. (2000). JBC. 275(4):2560-2567).

Calidad

Evaluated by Western Blotting in insulin treated NIH-3T3 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected IRAP in 10 µg of insulin treated NIH-3T3 cell lysate.

Descripción de destino

~150 kDa observed. The calculated molecular weight of this protein is 117 kDa, but can be observed at ~150 kDa due to high glycosylation. Uncharacterized band(s) may appear in some lysates.

Forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable