MABN1802

Anti-pan-PMCA Antibody, clone 5F10

clone 5F10, from mouse

• Sinónimos: Plasma membrane calcium-transporting ATPase, Plasma membrane calcium pump
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 5F10, monoclonal
• reactividad de especies: chicken, rat, human
• envase: antibody small pack of 25 μg
• técnicas: ELISA: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG2aκ
• Nº de acceso NCBI: NP_001001323.1; NP_001001331; NP_001001396.1; NP_068768.2
• Nº de acceso UniProt: P20020; P23634; Q01814; Q16720
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... ATP2B1(490) , ATP2B3(492) , ATP2B4(493)

Descripción

Descripción general

Plasma membrane calcium-transporting ATPase (isoforms 1-4) UniProt: P20020, Q01814, Q16720, P23634; also known as PMCA, Plasma membrane calcium pump) is encoded by the ATP2B1, ATP2B2, ATP2B3, ATP2B4 genes (Gene ID: 490; 491; 492; 493) in human. PMCAs are multi-pass membrane proteins that act as a calcium/calmodulin-regulated and magnesium-dependent enzymes and catalyze the hydrolysis of ATP coupled with the transport of calcium out of the cell. PMCAs serve as the main off switch for calcium signals in all types of cells. Although there are four separate genes that code for PMCAs 1-4, alternative splicing of the primary transcripts further increases the number of PMCA variants to about 20, which differ significantly in their regulatory and kinetic properties. PMCA activity is shown to be regulated at (a) the transcriptional level (b) by calmodulin binding and (c) by their subcellular localization. The localization of different PMCAs is regulated in various complex, cell type-specific ways. PMCA1 is ubiquitously expressed, PMCA2 is mainly expressed in brain cortex, but lower levels are also found in skeletal muscle, heart muscle, stomach, liver, kidney and lung. PMCA3 is highly expressed in the cerebellum, particularly in the presynaptic terminals of parallel fibers-Purkinje neurons. PMCA4 is expressed ubiquitously, however, its tissue distribution varies with its isoforms. (Ref.: Antalffy, G et al. (2013). Biochim. Biophys. Acta 1833(12); 2561-2572; Strehler, EE (2015). Biochem. Biophys. Commun. 460 (1); 26-33)

Especificidad

Clone 5F10 detects all isoforms of Plasma membrane calcium-transporting ATPase (PMCA) in human, mouse, and rat.

Inmunógeno

Purified human erythrocyte calcium ATPase.

Aplicación

Anti-pan-PMCA, clone 5F10, Cat. No. MABN1802, is a mouse monoclonal antibody that targets pan-Plasma membrane calcium-transporting ATPase (PMCA) and has been tested in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected pan-PMCA in human pancreas and human kidney tissue.

Immunocytochemistry Analysis: A representative lot detected pan-PMCA in Immunocytochemistry applications (Paszty, K., et. al. (2005). Biochem J. 391(Pt 3):687-92; DeMarco, S.J., et. al. (2002) J Biol Chem. 277(12):10506-11).

Immunoprecipitation Analysis: A representative lot immunoprecipitated pan-PMCA in Immunoprecipitation applications (DeMarco, S.J., et. al. (2002) J Biol Chem. 277(12):10506-11).

Western Blotting Analysis: A representative lot detected pan-PMCA in Western Blotting applications (Paszty, K., et. al. (2005). Biochem J. 391(Pt 3):687-92; Caride, A.J., et. al. (1996). Biochem J. 316 ( Pt 1):353-9).

ELISA Analysis: A representative lot detected pan-PMCA in ELISA applications (Caride, A.J., et. al. (1996). Biochem J. 316 ( Pt 1):353-9).

Research Category
Neuroscience

Calidad

Evaluated by Immunohistochemistry (Paraffin) in human cerebral cortex tissue

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected pan-PMCA in human cerebral cortex tissue sections.

Descripción de destino

134 to 139 kDa calculated.

Forma física

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash