MABN1616

Anti-GADD153/DDIT3/CHOP Antibody, clone 5E5.3

clone 5E5.3, from mouse

• Sinónimos: DNA damage-inducible transcript 3 protein, CCAAT/enhancer-binding protein homologous protein, C/EBP-homologous protein 10, C/EBP zeta, CHOP, CHOP-10, DDIT-3, Growth arrest and DNA damage-inducible protein GADD153
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 5E5.3, monoclonal
• reactividad de especies: human
• técnicas: immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_001181986
• Nº de acceso UniProt: P35638
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CEBPZ(10153)

Descripción

Descripción general

DNA damage-inducible transcript 3 protein (UniProt P35638; also known as CCAAT/enhancer-binding protein homologous protein, C/EBP-homologous protein 10, C/EBP zeta, CHOP, CHOP-10, DDIT-3, Growth arrest and DNA damage-inducible protein GADD153) is encoded by the DDIT3 (Also known as CEBPZ, CHOP, CHOP10, GADD153) gene in human (Gene ID 1649). CHOP is a proapoptotic unfold protein response (UPR) gene and a well established marker for ER stress and UPR activation. The accumulation of unfolded or misfolded proteins in the ER induces the activation of three main UPR pathways, the inositol requiring enzyme-1 (IRE1) pathway, the activating transcription factor 6 (ATF6) pathway, and the protein kinase-like ER kinase (PERK) pathway. The PERK pathway involves PERK auto-phosphorylation/activation, resulting in PERK phosphorylation of eIF2α, which in turn leads to increased ATF4 transcription and ATF4-mediated CHOP upregulation.

Especificidad

Clone 5E5.3 targeted region is present in both human GADD153/DDIT3/CHOP spliced isoforms reported by UniProt (P35638).

Inmunógeno

Epitope: Internal.

GST-tagged recombinant human GADD153/DDIT3/CHOP internal fragment.

Aplicación

Immunocytochemistry Analysis: 5.0 µg/mL from a representative lot detected GADD153/DDIT3 in HeLa cells.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected GADD153/DDIT3 in human brain, kidney, and skeletal muscle tissue sections.

Research Category
Neuroscience

Research Sub Category
Apoptosis - Additional

This Anti-GADD153 Antibody, clone 5E5.3 is validated for use in Western Blotting, Immunocytochemistry, Immunohistochemistry (Paraffin) for the detection of CHOP.

Calidad

Evaluated by Western Blotting Analysis in HUVEC lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected GADD153/DDIT3 in 10 µg of HUVEC lysate.

Descripción de destino

~20 kDa observed. 19.18 kDa (isoform 1) and 21.70 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

Forma física

Format: Purified

Protein G purified.

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable