MABN1596

Anti-Pan Shc Antibody, clone 3A12.1

clone 3A12.1, from mouse

• Sinónimos: p46Shc, p52Shc, p66Shc, SHC-transforming protein 1, SH2 domain protein C1, SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 3A12.1, monoclonal
• reactividad de especies: human
• técnicas: immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG2aκ
• Nº de acceso NCBI: NP_001123512
• Nº de acceso UniProt: P29353
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... SHC1(6464)

Descripción

Descripción general

SHC-transforming protein 1 (UniProt P29353; also known as SH2 domain protein C1, SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1) is encoded by the SHC1 (also known as SHC, SHCA) gene (Gene ID 6464) in human. Originally identified as an SH2-containing proto-oncogene involved in growth factor signaling, Shc proteins function as adaptor molecules recruited by numerous receptors (e.g. integrins, GPCRs, and receptors for growth factors, antigens, cytokines, and hormones) for signling. In addition to the ubiquitously expressed Shc (a.k.a. ShcA by SHC1/SHCA gene), there exist also ShcB (by SHC2/SHCB gene) and ShcC (by SHC3/SHCC gene), which are predominantly expressed in neuronal cells. Alternative splicings result in six ShcA isoforms, including p66Shc, p52Shc, and p46Shc. All ShcA isoforms possess two distinct domains, PTB (a.a. 156-339) and SH2 (a.a. 488-579), that bind phosphotyrosine-containing sequences, as well as a central region (CH1; a.a. 340-487) that contains critical tyrosine phosphorylation sites. The phosphotyrosine-binding (PTB) domain is also referred to as phosphotyrosine-interaction domain or PID.

Especificidad

Clone 3A12.1 was raised against a C-terminal region sequence present in all six human SHC1 spliced isoforms reported by UniProt (P29353).

Inmunógeno

GST-tagged recombinant human Shc C-terminal region fragment.

Aplicación

Anti-Pan Shc Antibody, clone 3A12.1 is an antibody against Pan Shc for use in Western Blotting, Immunohistochemistry.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Shc proteins in human prostate cancer, as well as in normal breast and tonsil tissue sections.

Calidad

Evaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Shc proteins in 10 µg of SH-SY5Y cell lysate.

Descripción de destino

~47-63 kDa observed. 62.82 kDa (isoform p66Shc), 51.61 kDa (isoform p52Shc), 46.67 kDa (isoform p46Shc), 40.42 kDa (isoform 5), 62.89 kDa (isoform 6), 51.68 kDa (isoform 7) calculated.

Forma física

Format: Purified

Otras notas

Concentration: Please refer to lot specific datasheet.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable