Saltar al contenido
Merck
Todas las fotos(2)

Key Documents

MABN1194

Sigma-Aldrich

Anti-PSD95 Antibody, clone 3H4.3

clone 3H4.3, from mouse

Sinónimos:

Disks large homolog 4, PSD95, Postsynaptic density protein 95, SAP90

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

3H4.3, monoclonal

reactividad de especies

mouse, rat, human

técnicas

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotipo

IgG1κ

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

ambient

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... DLG4(1742)
rat ... Dlg4(29495)

Descripción general

Disks large homolog 4 (UniProt: P31016; also known as Postsynaptic density protein 95, PSD-95, Synapse-associated protein 90, SAP-90, SAP90) is encoded by the Dlg4 (also known as Dlgh4, Psd95) gene (Gene ID: 29495) in rat species. PSD95 belong to the family of membrane-associated guanylate kinases (MAGUKs), which also include PSD-93, SAP97 and SAP102. PSD95 is predominantly expressed in the hippocampus CA1 region and prefrontal cortex of the brain, where it localizes in a somatodendritic pattern in the post-synaptic membrane and in presynaptic axon terminals of inhibitory neurons. PDS95 is the major scaffolding protein in the excitatory postsynaptic density (PSD) and a potent regulator of synaptic strength via interactions with a diverse PSD proteins, including NMDA receptors (NMDARs), AMPA receptor (AMPAR) complexes via Stargazin/TARP, adhesion molecules, as well as other scaffolding proteins, such as GKAP and Shank. PSD95 and neuronal nitric-oxide synthase (nNOS) interaction plays a role in maintaining hypersensitivity in acute and chronic pain. The assembly of the GluR6-PSD95-CaMKII complex is reported to mediate injury induced by brain ischemia by facilitating GluR6 serine phosphorylation by CaMKII and the activation of JNK. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons.

Especificidad

Clone 3H4.3 detects PSD95 in human and mouse brain.

Inmunógeno

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of rat PSD95 protein.

Aplicación

Anti-PSD95, clone 3H4.3, Cat. No. MABN1194, is a highly specific mouse monoclonal antibody that targets PSD95 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected PSD95 in human cerebellum, rat cerebellum, and rat brain stem tissues.
Research Category
Neuroscience

Calidad

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PSD-95 in 10 µg of mouse brain tissue lysate.

Descripción de destino

~95 kDa observed; 80.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forma física

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

¿No encuentra el producto adecuado?  

Pruebe nuestro Herramienta de selección de productos.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico