MABN1188

Anti-Tyrosine Hydroxylase Antibody, clone 10F8.2

clone 10F8.2, from mouse

• Sinónimos: TH, Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, Tyrosine Hydroxylase
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 10F8.2, monoclonal
• reactividad de especies: human, rat
• reactividad de especies (predicha por homología): mouse (based on 100% sequence homology)
• técnicas: immunohistochemistry: suitable (paraffin); western blot: suitable
• isotipo: IgG2bλ
• Nº de acceso NCBI: NP_036872
• Nº de acceso UniProt: P04177
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... TH (7054)

Descripción

Descripción general

Tyrosine 3-monooxygenase (EC 1.14.16.2; UniProt P04177; also known as TH, Tyrosine 3-hydroxylase) is encoded by the Th gene (Gene ID 25085) in rat species. Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines, dopamine and norepinephrine, from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. TH enzymatic activity requires ferrous ions as cofactor and is believed to be regulated by phosphorylation. Six human TH isoforms exist as a result of alternative splicing (UniProt P07101-1 through P07101-6).

Especificidad

Clone 10F8.2 targets a C-terminal epitope present in rat and mouse TH sequence (UniProt P04177 & P24529), as well as in all six human TH spliced isoforms (UniProt P07101).

Inmunógeno

Epitope: C-terminal region.

KLH-conjugated linear peptide corresponding to a C-terminal region sequence of rat tyrosine hydroxylase.

Aplicación

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected tyrosine hydroxylase in rat brain tissue sections.

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

This Anti-Tyrosine Hydroxylase Antibody, clone 10F8.2 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Tyrosine Hydroxylase.

Calidad

Evaluated by Western Blotting in PC12 cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected tyrosine hydroxylase in 10 µg of PC12 cell lysate.

Descripción de destino

~50-60 kDa observed. 55.83 kDa (rat), 55.86 kDa (mouse), 58.03/55.48/58.47/55.92/45.21/44.77 kDa (human isoform 1/2/3/4/5/6) calculated (Met1 removed). The multiplet banding pattern observed in rat PC12 lysate most likely represent differentially phosphorylated TH species (Gozal, E., et al. (2005). J. Appl. Physiol. 99(2):642-649).

Ligadura / enlace

Replaces: MAB318

Forma física

Format: Purified

Protein G purified.

Purified mouse monoclonal IgG2bλ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable