MABN109

Anti-Synaptotagmin-4 Antibody, clone 10C11.1

clone 10C11.1, from mouse

• Sinónimos: Synaptotagmin-4, Synaptotagmin IV, SytIV
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 10C11.1, monoclonal
• reactividad de especies: human, mouse
• técnicas: immunohistochemistry: suitable (paraffin); western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_065834
• Nº de acceso UniProt: Q9H2B2
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... SYT4(6860)

Descripción

Descripción general

Synaptotagmin-4 (UniProt Q9H2B2; also known as Synaptotagmin IV, SytIV) is encoded by the SYT4 (also known as KIAA1342) gene (Gene ID 6860) in human. The mammalian synaptotagmin family is comprised of 17 members. While most of them are predominantly function in the cetnral nervous system (CNS), some are involved in the vesicular functions of endocrine cells and glucose-transport metabolic cells. Synaptotagmin-4 is an inducible member detectable only in the brain and the neuroendocrine system. Unlike other family members, synaptotagmin-4 does not bind calcium and does not medidate calcium-induced exocytosis. Instead, synaptotagmin-4 is shown to play a neuroendocrine role in inhibiting exocytotic activities in the posterior pituitary and in cultured PC12 cells. Synaptotagmin-4 is expressed most abundantly in oxytocin neurons of the hypothalamus, where it negatively regulates oxytocin exocytosis. Dietary obesity is associated with increased vesicle binding of synaptotagmin-4 and decreased oxytocin release. Normalizing oxytocin release via Synaptotagmin-4 inhibition prevents against dietary obesity under chronic nutritional excess. Human synaptotagmin-4 consists of a vesiclular domain (a.a. 1-16), a transmembrane segment (a.a. 17-37),and a large cytoplasmic domain (a.a. 38-425).

Especificidad

Clone 10C11.1 targets a cytoplasmic epitope present in both human spliced isoforms reported by UniProt (Q9H2B2).

Inmunógeno

Epitope: Cytoplasmic domain.

GST-tagged recombinant human Synaptotagmin-4 cytoplasmic domain fragment.

Aplicación

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Synaptotagmin-4 in human kidney, skeletal muscle, and pancreas tissue sections.

Research Category
Neuroscience

Research Sub Category
Vesicular Trafficking

This Anti-Synaptotagmin-4 Antibody, clone 10C11.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Synaptotagmin-4.

Calidad

Evaluated by Western Blotting in mouse cerebellum tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Synaptotagmin-4 in 10 µg of mouse cerebellum tissue lysate.

Descripción de destino

~48 kDa observed. 47.96/46.06 kDa (human isoform 1/2) and 47.66 kDa (mouse) calculated.

Forma física

Format: Purified

Protein G purified.

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1