MABF568

Anti-CD4 Antibody (mouse), violetFluor^® 450, clone GK1.5

clone GK1.5, 0.2 mg/mL, violetFluor^®

• Sinónimos: T-cell surface glycoprotein CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, CD4
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rat
• Nivel de calidad: 100
• conjugado: violetFluor^® 450
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: GK1.5, monoclonal
• reactividad de especies: mouse
• fabricante / nombre comercial: violetFluor^®
• concentración: 0.2 mg/mL
• técnicas: flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• isotipo: IgG2bκ
• Nº de acceso UniProt: P06332
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: mouse ... Cd4(12504)

Descripción

Descripción general

The GK1.5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The GK1.5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the GK1.5 antibody and an alternative antibody, Anti-Mouse CD4 clone RM4-5, will “compete” for binding, i.e. RM4-5 is able to block GK1.5 binding to cells. In contrast, the Anti-Mouse CD4 clone RM4-4 does not block binding of the GK1.5 antibody to cells (Arora S et al. 2006. Infect. Immun. 74: 4339-4348). The GK1.5 antibody is also reported to be cross-reactive with Syrian hamster CD4.

This rat monoclonal antibody Anti-CD4 (mouse), violetFluor 450, clone GK1.5 validated for use in IP, IHC & Flow Cytometry for the detection of CD4.

Calidad

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 µg of this antibody detected CD4 in one million C57Bl/6 splenocytes.

Forma física

Purified 0 Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.

Información legal

violetFluor is a registered trademark of Tonbo Biotechnologies

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable