MABF3181

Anti-IL-4 Antibody, clone 2D7.14.8

• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 2D7.14.8, monoclonal
• mol peso: calculated mol wt 17.49 kDa; observed mol wt ~15 kDa
• purificado por: using protein G
• reactividad de especies: human
• envase: antibody small pack of 100 μg
• técnicas: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotipo: IgG2aκ
• secuencia del epítopo: Unknown
• Nº de acceso Protein ID: NP_000580.1?
• Nº de acceso UniProt: P05112
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: mouse ... IL4(3565)

Descripción

Descripción general

Interleukin-4 (UniProt: P05112; also known as IL-4, B-cell stimulatory factor 1, BSF-1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra) is encoded by the IL4 gene (Gene ID: 3565) in human. IL-4 is a cytokine that is secreted primarily by mast cells, Th2 cells, eosinophils, and basophils. IL-4 is synthesized with a signal peptide (1-24), which is subsequently cleaved off to produce the mature form. It participates in the activation of B cells and other cell types. It also serves as a co-stimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also reported to regulate the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. IL-4 inhibits macrophage production of proinflammatory cytokines, including TNF-a, IL-1, and IL-6 via transcriptional repression. It also enhances production of molecules that counteract these cytokines, such as IL-1R antagonist and the decoy receptor IL-1RII. The effects of IL-4 signaling are mediated through binding to the IL-4 receptor a chain (IL-4R ) that dimerizes with g-chain upon ligand binding to produce type I signaling complex located mainly of hematopoietic cells. It can also dimerize with IL-13a receptor a (IL-13Ra1) to produce type-2 complex that is expressed also on non-hematopoietic cells. IL-4 is also shown to stimulate autophagy in dendritic cells by interfering with mTORC1 signaling through the induction of RUN and FYVE domain containing 4 (RUFY4). (Ref.: Gadani, SP., et al. (2012). J. Immunol. 189(9); 4213-4219; Otsuka, TY., et al. (1986). Proc. Natl. Acad. Sci. USA. 83(16); 5894-5898).

Especificidad

Clone 2D7.14.8 is a mouse monoclonal antibody that detects human Interleukin-4.

Inmunógeno

Full-length recombinant human Interleukin-4 (without the signal peptide).

Aplicación

Quality Conrol Testing

Evaluated by Western Blotting with recombinant human IL-4

Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected recombinant human IL-4.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-4 in human tonsil tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-IL-4, clone 2D7.14.8, Cat. No. MABF3181, is a mouse monoclonal antibody that detects human Inerleukin-4 and is is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Forma física

Purified mouse monoclonal antibody IgG2a in PBS with 0.05% sodium azide.

Almacenamiento y estabilidad

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable