MABF3036

Anti-PSGL-1/CD162 Antibody, clone HECA-452

• Sinónimos: P-selectin glycoprotein ligand 1;PSGL-1 Selectin P ligand,;CD162
• Código UNSPSC: 12352203

Propiedades

• origen biológico: rat
• Nivel de calidad: 200
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: HECA-452, monoclonal
• mol peso: calculated mol wt 43.2 kDa
• purificado por: affinity chromatography
• reactividad de especies: human
• envase: antibody small pack of 100 μg
• técnicas: flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotipo: IgMκ
• secuencia del epítopo: Extracellular domain
• Nº de acceso Protein ID: NP_001193538
• Nº de acceso UniProt: Q14242
• temp. de almacenamiento: -10 to -25°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... SELPLG(6404)

Descripción

Descripción general

P-selectin glycoprotein ligand 1 (UniProt: Q14242; also known as PSGL-1 Selectin P ligand, CD162) is encoded by the SELPLG gene (Gene ID: 6404) in human. Leukocytes express L-selectin ligands that are critical for leukocyte-leukocyte interactions at sites of inflammation. PSGL-1 is a predominant leukocyte L-selectin ligand that displays appropriate sialyl Lewis x (sLex)-like carbohydrate determinants for L-selectin recognition. It is a single-pass, mucin-like type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off to produce a form that contains an extracellular domain (18-320), a transmembrane domain (321-341), and a cytoplasmic domain (342-412). The mature form is generated following the cleaved of the propeptide (aa 18-41) region. PSGL-1 is a SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. It is constitutively expressed on all human peripheral-blood T cells and is also expressed on skin-homing T cells. Clone HECA-452 can detect a human endothelial cell differentiation antigen (HECA) selectively expressed on high endothelium. In addition to reacting with high endothelium, it also shows some cross-reactivity with a set of monocytic cells. This clone is shown to block the majority of P-selectin binding to neutrophils. (Ref.: Tu, L., et al. (1999). J. Immunol. 163(9); 5070-5078; Fuhlbrigge, RC., et al. (1997). Nature. 389(6654); 978-981; Duivestijn, AM., et al. (1988). Am. J. Pathol. 130(1); 147-155).

Especificidad

Clone HECA-452 is a rat monoclonal antibody that detects PSGL-1/CD162. It targets an epitope within the extracellular domain.

Inmunógeno

Stromal preparation from human tonsil tissue.

Aplicación

Quality Control Testing

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis (FC): 1.0 μg of this antibody stained one million Human peripheral blood mononuclear cells (PBMC).

Tested Applications

Immunohistochemistry Applications: A representative lot detected PSGL-1/CD162 in Immunohistochemistry application (Duijvestijn, A.M., et al. (1988). Am J Pathol. 130(1):147-55).

Flow Cytometry Analysis: A representative lot detected PSGL-1/CD162 in Flow Cytometry application (Picker, L.J., et al. (1991). Nature. 349(6312):796-9; Fuhlbrigge, R.C., et al. (1997). Nature. 389(6654):978-81).

Western Blotting Analysis: A representative lot detected PSGL-1/CD162 in Western Blotting application (Fuhlbrigge, R.C., et al. (1997). Nature. 389(6654):978-81).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Forma física

Purified rat monoclonal antibody IgM in PBS without azide.

Reconstitución

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable