MABF2816

Anti-Cortical stromal cells Antibody, clone ER-TR4

• Sinónimos: cTEC
• Código UNSPSC: 12352203
• NACRES: NA.43
• clon: ER-TR4, monoclonal
• application: IF; IHC
• reactividad de especies: mouse
• técnicas: immunofluorescence: suitable; immunohistochemistry: suitable

Propiedades

• origen biológico: rat
• Nivel de calidad: 200
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: ER-TR4, monoclonal
• purificado por: affinity chromatography
• reactividad de especies: mouse
• envase: antibody small pack of 100 μg
• técnicas: immunofluorescence: suitable; immunohistochemistry: suitable
• isotipo: IgMκ
• secuencia del epítopo: Unknown
• Nº de acceso Protein ID: N/A
• Nº de acceso UniProt: N/A
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

The thymus is considered as the primary lymphoid organ that is responsible for the generation and maturation of T cells. Thymic epithelial cells (TECs) account for the majority of thymic stromal components. These cells can be divided into cortical and medullary thymic epithelial cells based on their localization within the thymus. TECs have dual germ layer origins, with cortical cells arising from ectoderm and medullary cells arising from the endoderm. In an embryonic thymus, cortical cells are the dominant population, while medullary cells account for most of the TECs in the adult thymus. Cortical thymic epithelial cells are essential for the positive selection of T cells, whereas the medullary thymic epithelial cells (mTECS) play a role in inducing negative selection of highly reactive T cells that are required for establishing central self-tolerance. mTECs participate in self-tolerance by eliminating self-reactive T cells. Cortical cells that support positive selection also support post-selection maturation of single-positive thymocytes. They provide cytokines, such as IL-7 and SCF, to promote early thymic progenitors (ETP) cells and Delta-like ligand 4 (DLL4)-mediated Notch signaling to induce the differentiation of ETPs towards the T lineage. DLL4 is reported to be expressed at high levels in cortical cells, but not in medullary cells. Clone ER-TR4 reacts with epithelial-reticular cells in the cortex of murine thymus. (Ref.: Wang, HX., et al. (2020). Front. Immunol. 10; 3099; Van Vliet, E., et al. (1984). Eur. J. Immunol. 14(6); 524-529; Cordier, AC., et al. (1980). Am. J Anat. 157(3); 227-263).

Especificidad

Clone ER-TR4 is a rat monoclonal antibody that detects cortical stromal cells.

Inmunógeno

Thymic stromal cells isolated from C3H mice.

Aplicación

Quality Control Testing

Evaluated by Immunohistochemistry in mouse thymus tissue sections.

Immunohistochemistry Applications: A 1:50 dilution of this antibody detected Cortical stromal cells in mouse thymus tissue sections.

Tested applications

Immunofluorescence Analysis: A representative lot detected Cortical stromal cells in Immunofluorescence applications (Tanaka, Y., et al., (2007). Blood. 110(1):107-15).

Immunohistochemistry Applications: A representative lot detected Cortical stromal cells in Immunohistochemistry applications (Van Vliet, E., et al., (1984). Eur J Immunol. 14(6):524-9; Scheijen, B., et al., (2004). J Biol Chem. 279(11):10476-83; Dooley, J., et al., (2006). J Immunol. 176(11):6484-90; Tanaka, Y., et al., (2007). Blood. 110(1):107-15; Moretti, F.A., et al., (2018). Elife. 7:e35816; Hollander, G.A., et al. (1995). Nature. 373(6512):350-3).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Forma física

Purified rat monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitución

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommended storage: +2°C to +8°C.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable