MABF265

Anti-Caspase-4, clone 17D9 Antibody

clone 17D9, from rat

• Sinónimos: Caspase-4, CASP-4, Caspase-11, CASP-11, Protease ICH-3, .Caspase-4 subunit p10, Caspase-4 subunit p20
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rat
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 17D9, monoclonal
• reactividad de especies: human, mouse
• técnicas: immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG2aκ
• Nº de acceso NCBI: NP_031635
• Nº de acceso UniProt: P70343
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CASP4(837)

Descripción

Descripción general

Caspase-4 (CASP-4), also known as Caspase-11 (CASP-11), or Protease ICH-3, and encoded by the gene names Casp4, Casp11, Caspl, and Ich3, is a member of the peptidase C14A family and might be involved in ER-stress induced apoptosis. Caspase-4 protein has been shown to cleave Caspase-1 and might be involved in the activation cascade of caspases responsible for apoptosis execution. Caspase-4 promotes IL-1 beta processing by ICE, and therefore may also play a role in inflammatory responses. Caspase-4 has been indicated to interact with TMEM214, in the process of ER membrane localization. Additionally, Caspase-4 is a heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The two subunits are derived by an autocatalytic mechanism, from the precursor sequence. Recent studies have shown that the activity of Caspase-4 is increased 30-fold by endotoxins (LPS). Other studies have indicated that Caspase-4 is induced by ER stress in a DDIT3/CHOP-dependent manner. Caspase-4 is expressed mostly in lung and spleen, and less in liver, skeletal muscle, kidney and testis.

Inmunógeno

Corresponding to mouse Caspase-4.

Aplicación

Anti-Caspase-4, clone 17D9 is an antibody against Caspase-4 for use in WB, IP, IH.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Caspase-4 in ischemia induced mouse brains (Kang, S., et al. (2000) JCB. 149(3):613-622).
Immunohistochemistry Analysis: A representative lot detected Caspase-4 in wildtype and ischemic mouse brains (Kang, S., et al. (2000) JCB. 149(3):613-622).

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Calidad

Evaluated by Western Blotting in bacterial J53 treated macrophage cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Caspase-4 in 10 µg of bacterial J53 treated macrophage cell lysate.

Descripción de destino

~48 and 38 kDa observed

Forma física

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable