MABF2169

Anti-HLA-G Antibody, clone 4H84

clone 4H84, from mouse

• Sinónimos: HLA class I histocompatibility antigen alpha chain G, HLA G antigen, MHC class I antigen G
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 4H84, monoclonal
• reactividad de especies: human
• envase: antibody small pack of 25 μg
• técnicas: ELISA: suitable; flow cytometry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_002118.1
• Nº de acceso UniProt: P17693
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... HLA-G(3135)

Descripción

Descripción general

HLA class I histocompatibility antigen, alpha chain G (UniProt: P17693; also known as HLA G antigen, MHC class I antigen G) is encoded by the HLA-G (also known as HLA-6.0, HLAG) gene (Gene ID: 3135) in human. HLA-G is a single-pass type I membrane glycoprotein that serves as marker of extravillous cytotrophoblasts in placenta and plays a central role in molecule maternal tolerance of the semiallogeneic fetus. It is involved in the presentation of foreign antigens to the immune system. HLA-G is a heterodimer of an alpha chain and a beta chain. Severn different isoforms of HLA-G have been described that are produced by alternative splicing and their distribution is temporally affected during pregnancy in placenta and fetal tissues. HLA-G expression has been reported in renal cell carcinoma where it is exclusively restricted to tumor cells. Its expression is thought to be involved in the impairment of the anti-tumor immunity or resistance to immunotherapeutic agents. HLA-G is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to generate the mature form, which contains an extracellular domain (aa 25-308), a transmembrane domain (aa 309-332), and a short cytoplasmic tail (aa 333-338).

Especificidad

Clone 4H84 specifically detects human HLA-G. It recognizes an epitope with in 23 amino acids from the alpha1 region of all HLA-G isoforms.

Inmunógeno

KLH-conjugated linear peptide corresponding to 23 amino acids from the N-terminal region of Human MHC class I antigen G. The immunogen sequence is conserved in all isoforms.

Aplicación

Anti-HLA-G, clone 4H84, Cat. No. MABF2169, is a mouse monoclonal antibody that detects HLA class I histocompatibility antigen, alpha chain G and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected HLA-G in human spleen tissue sections.

Immunohistochemistry (Paraffin) Analysis: A representative lot detected HLA-G in Immunohistochemistry applications (Tabata, T., et. al. (2012). Am J Pathol. 181(5):1540-59; Menier, C., et. al. (2003). Hum Immunol. 64(3):315-26; Ibrahim, E.C., et. al. (2003). Am J Pathol. 162(2):501-8; Tabata, T., et. al. (2016). Cell Host Microbe. 20(2):155-66; McMaster, M., et. al. (1998). J Immunol. 160(12):5922-8).

Western Blotting Analysis: A representative lot detected HLA-G in Western Blotting applications (Paul, P., et. al. (1999). Cancer Res. 59(8):1954-60; McMaster, M., et. al. (1998). J Immunol. 160(12):5922-8).

ELISA Analysis: A representative lot detected HLA-G in ELISA applications (McMaster, M., et. al. (1998). J Immunol. 160(12):5922-8).

Flow Cytometry Analysis: A representative lot detected HLA-G in Flow Cytometry applications (Menier, C., et. al. (2003). Hum Immunol. 64(3):315-26).

Research Category
Inflammation & Immunology

Calidad

Evaluated by Immunohistochemistry (Paraffin) in human placenta tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected HLA-G in human placenta tissue sections.

Descripción de destino

38.22 kDa calculated.

Forma física

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.