MABF2067M

Anti-Calnexin Antibody, clone AF8

clone AF8, from mouse

• Sinónimos: IP90, Major histocompatibility complex class I antigen-binding protein p88, p90
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: AF8, monoclonal
• reactividad de especies: human
• envase: antibody small pack of 25 μg
• técnicas: flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_001019820.1
• Nº de acceso UniProt: P27824
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CANX(821)

Descripción

Descripción general

Calnexin (UniProt: P27824; also known as IP90, Major histocompatibility complex class I antigen-binding protein p88, p90) is encoded by the CANX gene (Gene ID: 821) in human. Calnexin is a well-conserved, calcium binding, endoplasmic reticulum (ER) resident chaperone protein that associates with newly synthesized, incompletely folded monomeric glycoproteins and promotes their correct folding prior to secretion. It is also reported to target misfolded proteins for degradation. It is anchored to the ER membrane by a transmembrane domain. Calnexin associates with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes and may, therefore, function as a signaling complex regulating thymocyte maturation. Calnexin is synthesized with a signal peptide of 20 amino acids, which is cleaved to generate the mature form. Three isoforms of calnexin have been described that are generated by alternative splicing. Calnexin is shown to be phosphorylated at Ser564 by MAPK3/ERK1, which increases its association with ribosomes.

Especificidad

Clone AF8 detects Calnexin in human cells.

Inmunógeno

TCR complexes isolated from the human T-cell leukemia line, DND41.

Aplicación

Anti-Calnexin, clone AF8, Cat. No. MABF2067, is a mouse monoclonal antibody that detects calnexin in human cells and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Flow Cytometry Analysis: 1 µg from a representative lot detected Calnexin in one million MCF7 cells.

Immunoprecipitation Analysis: A representative lot detected Calnexin in Immunoprecipitation applications (Hochstenbach, F., et. al. (1992). Proc Natl Acad Sci USA. 89(10):4734-8; Rajagopalan, S., et. al. (1994). J Exp Med. 180(1):407-12).

Immunohistochemistry Analysis: A representative lot detected Calnexin in Immunohistochemistry applications (Hochstenbach, F., et. al. (1992). Proc Natl Acad Sci USA. 89(10):4734-8).

Flow Cytometry Analysis: A representative lot detected Calnexin in Flow Cytometry applications (Rajagopalan, S., et. al. (1994). J Exp Med. 180(1):407-12).

Western Blotting Analysis: A representative lot detected Calnexin in Western Blotting applications (Hochstenbach, F., et. al. (1992). Proc Natl Acad Sci USA. 89(10):4734-8).

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected Calnexin in HeLa, A431 and Huvec cell lines.

Research Category
Inflammation & Immunology

Calidad

Evaluated by Immunocytochemistry in MCF7 cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Calnexin in MCF7 cells.

Descripción de destino

67.57 kDa calculated.

Ligadura / enlace

Replaces: MAB3126

Forma física

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable