MABF1988

Anti-CMTM6 Antibody, clone RCT6

• Sinónimos: CKLF-like MARVEL transmembrane domain-containing protein 6;Chemokine-like factor superfamily member 6
• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: RCT6, monoclonal
• mol peso: calculated mol wt 20.42 kDa; observed mol wt ~20 kDa
• purificado por: using protein G
• reactividad de especies: human
• envase: antibody small pack of 100 μg
• técnicas: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotipo: IgG1κ
• secuencia del epítopo: C-terminal
• Nº de acceso Protein ID: NP_060271
• Nº de acceso UniProt: Q9NX76
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CMTM6(54918)

Descripción

Descripción general

CKLF-like MARVEL transmembrane domain-containing protein 6 (UniProt: Q9NX76; also known as Chemokine-like factor superfamily member 6, CMTM6) is encoded by the CMTM6 gene (Gene ID: 54918) in human. CMTM6 is a ubiquitously expressed, multi-pass membrane protein of the family of MARVEL domain-containing proteins with 3 cytoplasmic domains, 4 transmembrane domains, and 2 extracellular domains. Its MARVEL domain is localized in amino acids 33-160. It serves as a master regulator of recycling and plasma membrane expression of PD-L1. It binds to PD-L1 and maintains its cell surface expression. Although it is not required for PD-L1 maturation, but it co-localizes with PD-L1 at the plasma membrane and in recycling endosomes, where it prevents PD-L1 from being targeted for lysosome-mediated degradation by preventing its STUB-1 mediated ubiquitination. It also stabilizes PD-L1 expression on antigen presenting cells and potentiates inhibitory signaling by PD-1 (CD279), ultimately triggering T-cell anergy. It enhances the ability of PD-L1-expressing tumor cells to inhibit T cell activity. (Ref.: Burr, ML., et al. (2017). Nature. 549(7670); 101-105; Mezzadra, R., et al. (2017). Nature. 549(7670); 106-110).

Especificidad

Clone RCT6 is a mouse monoclonal antibody that detects human CMTM6. It targets an epitope within the C-terminal region.

Inmunógeno

A linear peptide corresponding to a sequence from the C-terminal region of human CKLF-like MARVEL transmembrane domain-containing protein 6 (CMTM6).

Aplicación

Quality Control Testing

Evaluated by Western blotting in lysates from 8505c cells overexpressing CMTM6.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected CMTM6 in lysates from 8505C cells overexpressing CMTM6, but not in knockout or untransfected cells.

Tested Applications

Immunohistochemistry Applications: A representative lot detected CMTM6 in Immunohistochemistry applications (Mezzadra, R., et al. (2017). Nature. 549(7670):106-110).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-CMTM6, clone RCT6, Cat. No. MABF1988, is a mouse monoclonal antibody that detects CMTM6 and is tested for use in Immunohistochemistry and Western Blotting.

Forma física

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Recommended storage: +2°C to +8°C.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable