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Key Documents

MABF161B

Sigma-Aldrich

Anti-CD45 (human), redFluor® 710, clone HI30 Antibody

clone HI30, from mouse, redFluor® 710

Sinónimos:

Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen (L-CA), Lymphocyte antigen 5 (Ly-5), T200, CD45, CD45

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

conjugado

redFluor® 710

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

HI30, monoclonal

reactividad de especies

human

técnicas

flow cytometry: suitable

Nº de acceso UniProt

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... PTPRC(5788)

Descripción general

The HI30 antibody reacts with human CD45, one of the most abundant hematopoietic markers and one that is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, CD45RO and others.The HI30 antibody is widely used as a marker for human CD45 expression on T cells, B cells, monocytes, macrophages, and NK cells.

Inmunógeno

Immunogen information for this antibody is proprietary information.

Aplicación

Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This mouse monoclonal Anti-CD45 Antibody (human), redFluor® 710, clone HI30, Cat. No. MABF1613 is validated for use in Flow Cytometry, for the detection of CD45.

Calidad

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 μg from a representative lot detected CD45 in one million human peripheral blood lymphocytes.

Forma física

Purified Mouse monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Purified by size-exclusion chromatography

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Otras notas

Concentration: Please refer to lot specific datasheet.

Información legal

redFluor is a registered trademark of Tonbo Biotechnologies

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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