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Key Documents

MABF153A

Sigma-Aldrich

Anti-F4/80 (mouse), PerCP-Cy5.5, clone BM8.1 Antibody

clone BM8.1, from rat, PerCP-Cy5.5

Sinónimos:

EGF-like module-containing mucin-like hormone receptor-like 1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EMR1 hormone receptor, F4/80

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

rat

Nivel de calidad

conjugado

PerCP-Cy5.5

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

BM8.1, monoclonal

reactividad de especies

mouse

envase

antibody small pack of 25 μg

técnicas

flow cytometry: suitable

isotipo

IgG2aκ

Nº de acceso UniProt

modificación del objetivo postraduccional

unmodified

Información sobre el gen

mouse ... Adgre1(13733)

Descripción general

The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. F4/80 is differentially expressed during myeloid cell development, and may be regulated by certain cytokines within the tissue microenvironment. Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry.

Inmunógeno

Immunogen information for this antibody is proprietary information.

Aplicación

Anti-F4/80 (mouse), PerCP-Cy5.5, clone BM8.1 Antibody is an antibody against F4/80 for use in Flow Cytometry.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

Calidad

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 μg from a representative lot detected F4/80 in one million C57Bl/6 bone marrow cells stained with FITC Anti-mouse CD11b.

Forma física

Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Purified by size-exclusion chromatography

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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