MABF1478

Anti-IFNGR1/CD119 Antibody, clone 2E2

• Sinónimos: CD119, IFN-gamma receptor 1, IFN-gamma-R-alpha, IFN-gamma-R1, Interferon gamma receptor 1, Interferon gamma receptor alpha-chain
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: hamster
• Nivel de calidad: 200
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 2E2, monoclonal
• mol peso: calculated mol wt 52 kDa; observed mol wt ~N/A kDa
• purificado por: using protein G
• reactividad de especies: mouse
• envase: antibody small pack of 100
• técnicas: flow cytometry: suitable
• isotipo: IgG2
• secuencia del epítopo: Extracellular domain
• Nº de acceso Protein ID: NP_034641
• Nº de acceso UniProt: P15261
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: mouse ... Ifngr1(15979)

Descripción

Especificidad

Clone 2E2 is an Armenian hamster monoclonal antibody that detects Interferon 1 receptor (IFNGR1/CD119). It targets an epitope within the extracellular domain.

Inmunógeno

Recombinant soluble form of -chain of mouse IFNGR1/CD119.

Aplicación

Quality Control Testing

Evaluated by Flow Cytometry in C57BL/6 Mouse splenocytes.

Flow Cytometry Analysis: 1.0 µg of this antibody detected IFNGR1/CD119 in one million C57BL/6 Mouse splenocytes.

Tested Applications

Flow Cytometry Analysis: A representative lot detected IFNGR1/CD119 in Flow Cytometry applications (Haring, J.S., et al. (2005). J Immunol. 174(11):6791-802).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

Interferon gamma receptor 1 (UniProt: P15261; also known as IFN-gamma receptor 1, IFN-gamma-R1, Interferon gamma receptor alpha-chain, IFN-gamma-R-alpha, CD119) is encoded by the Ifngr1 (also known as Ifngr) gene (Gene ID: 15979) in murine species. IFNGR1/CD119 is single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 26-254), a transmembrane domain (aa 255-275), and a cytoplasmic domain (aa 276-477). It serves as a receptor subunit for interferon- that plays critical roles in antibacterial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. It associates with IFNGR2 to form a functional receptor. Upon ligand binding, the intracellular domain of IFNGR1/CD119 opens out to allow association of downstream signaling components JAK1 and JAK2. In turn, the activated JAK1 phosphorylates IFNGR1/CD119 to form a docking site for STAT1. Subsequent phosphorylation of STAT1 leads to its dimerization, translocation to the nucleus, and stimulation of target gene transcription. Phosphorylation of the intracellular domain of IFNGR1/CD119 also provides a docking site for SOCS1 that regulates the JAK-STAT pathway by competing with STAT1 binding to IFNGR1. (Ref.: Wei, B., et al. (2010). Am. J. Transplant. 10(1); 69-80; Haring, JS., et al. (2005). J. Immunol. 174(11); 6791-6802; Qing, Y., et al. (2005). J. Biol. Chem. 280(3); 1849-1853).

Forma física

Purified armenian hamster monoclonal antibody IgG2-IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitución

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommended storage: +2°C to +8°C.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable