MABE8

Anti-EBNA2 Antibody, clone R3

clone R3, from rat

• Sinónimos: Epstein-Barr nuclear antigen 2
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• clon: R3, monoclonal
• application: IHC; IP; WB
• reactividad de especies: human
• técnicas: immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• citations: 13

Propiedades

• origen biológico: rat
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: R3, monoclonal
• reactividad de especies: human
• técnicas: immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG2aκ
• Nº de acceso NCBI: YP_001129441
• Nº de acceso UniProt: Q69022
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: Epstein-Barr virus ... Ebna-2(3783761)

Descripción

Descripción general

Gross, H., et al. (2010). Virology. 397(2): 299-310.
PMID: 19969318
Kremmer, E., et al. (1995). Virology. 208(1): 336-342.
PMID: 11831716

The Epstein-Barr virus or EBV is associated with several human cancers and actively immortalizes B lymphocytes. EBV is leads to expression by a small group of viral proteins, including Epstein-Barr nuclear antigen 2 (EBNA2) which is required for immortalization. EBNA2 is a transcriptional activator belonging to the herpesviridae EBNA2 family, and performs a crucial role in activating the host resting B-cell and inducing B-cell proliferation. EBNA2 possesses an acidic activating domain with which it is able to recruit various cellular and viral involved in B-cell immortalization. Implicated in cellular phenotypic modifications, EBNA2 is known to upregulate the expression of cell surface receptors, such as CD21, MYC, and CD23, as well as upregulating viral genes such as LMP1, LMP2A, LMP2B, and EBNA1-6.

Especificidad

Demonstrated to react with human cell infected with EBV.

Inmunógeno

Epitope: C-terminus of EBNA2

GST-tagged recombinant protein corresponding to human EBNA2.

Aplicación

Anti-EBNA2 Antibody, clone R3 is a Rat Monoclonal Antibody for detection of EBNA2 also known as Epstein-Barr nuclear antigen 2 & has been validated in WB, IP & IHC.

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Gross, H., et al. (2010). Virology. 397(2):299-310.)

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Kremmer, E., et al. (1995). Virology. 208(1):336-342.)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Calidad

Evaluated by Western Blot in B95-8 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected EBNA2 on 10 µg of B95-8 cell lysate.

Descripción de destino

~ 75 kDa observed; Although the calculated molecular weight is 49 kDa, this protein is typically observed at ~ 75-80 kDa

Forma física

Format: Purified

Protein G

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
B95-8 cell lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable