MABE2005

Anti-RNF4 Antibody, clone 11-3.1

• Sinónimos: E3 ubiquitin-protein ligase RNF4, Protein SNURF, RING finger protein 4, Small nuclear ring finger protein
• Código UNSPSC: 12352203

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 11-3.1, monoclonal
• mol peso: calculated mol wt 21.32 kDa; observed mol wt ~32 kDa
• purificado por: using protein G
• reactividad de especies: human
• envase: antibody small pack of 100
• técnicas: immunocytochemistry: suitable; western blot: suitable
• isotipo: IgG2bκ
• secuencia del epítopo: Unknown
• Nº de acceso Protein ID: NP_001171938
• Nº de acceso UniProt: P78317
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: human ... RNF4(6047)

Descripción

Especificidad

Clone 11-3.1 is a mouse monoclonal antibody that specifically detects recombinant human RNF4 protein.

Inmunógeno

GST-tagged, recombinant human RNF4 protein.

Aplicación

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution from a representative lot detected RNF4 protein in lysate from HeLa cells.

Tested Applications

Immunocytochemistry Analysis: A representative lot detected RNF4 in Immunocytochemistry application (Saito, M., et al. (2014). Biochem Biophys Res Commun. 447(1):83-8).

Western Blotting Analysis: A representative lot detected RNF4 in Western Blotting application (Saito, M., et al. (2014). Biochem Biophys Res Commun. 447(1):83-8).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

E3 ubiquitin-protein ligase RNF4 (UniProt: P78317; also known as RING finger protein 4, EC:2.3.2.27, Small nuclear ring finger protein, Protein SNURF) is encoded by the RNF4 (also known as SNURF) gene (Gene ID: 6047) in human. RNF4 gene encodes ubiquitin E3 ligase, an enzyme widely expressed in many tissues. RNF4 is mapped to human chromosome 4p16.3. The protein contains a RING-type zinc finger domain and around four small ubiquitin-related modifier (SUMO)-interaction motifs. The RING-type zinc finger domain is required for the ubiquitination of polysumoylated substrates and the SUMO interaction motifs (SIMs) mediates the binding to polysumoylated substrate. RNF4 is predominantly localized in the nucleus but can also be found in the cytoplasm. This dual localization allows it to function in multiple cellular compartments, dealing with SUMOylated substrates wherever they are located. The movement between these compartments can be regulated by specific signals or stress conditions. RNF4 is broadly expressed across various tissues, with notable levels in tissues that have high cellular turnover or are particularly responsive to stress, such as in immune cells and testis. It is involved in DNA repair, in transcription and chromatin regulation. It is also associated with the process of hematopoiesis. RNF4 is implicated in acute promyelocytic leukemia, acute myeloid leukemia, Fanconi anemia and aplastic anemia. Its role in maintaining protein homeostasis and responding to cellular stress makes it a key player in health and disease, with its dysfunction being linked to cancer, neurodegeneration, and developmental issues. Understanding the mechanisms regulating RNF4 activity and localization can provide insights into therapeutic strategies for these diseases. (Ref.: Saito, M., et al. (2014). Biochem Biophys Res Commun. 447(1):83-8. (PMID: 24695317).

Forma física

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitución

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommended storage: +2°C to +8°C.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable