MABE1934

Anti-IKFZ3 degron tag Antibody, clone 1C3-D3-F8

• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 1C3-D3-F8, monoclonal
• mol peso: calculated mol wt 58.02 kDa; observed mol wt ~39 kDa
• purificado por: using protein G
• reactividad de especies: human
• reactividad de especies (predicha por homología): monkey, bovine, rat
• envase: antibody small pack of 100 μL
• técnicas: western blot: suitable
• isotipo: IgG1κ
• secuencia del epítopo: N-terminal half
• Nº de acceso Protein ID: NP_001244337
• Nº de acceso UniProt: Q9UKT9
• Condiciones de envío: 2-8°C
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

Zinc finger protein Aiolos (UniProt: Q9UKT9; also known as Ikaros family zinc finger protein 3, IKZF3) is encoded by the IKZF3 (also known as ZNFN1A3) gene (Gene ID: 22806) in human. IKZF3 is a member of the Ikaros Zinc finger family of transcription factors with six C2H2 type zinc finger domains. It is expressed mainly in peripheral blood leukocytes, the spleen, and the thymus. It is present as a homodimer and can also form heterodimers with other Ikaros family members. It plays an important role in the regulation of lymphocyte differentiation and is involved in the regulation of B-cell differentiation, proliferation and maturation. It is also shown to regulate Bcl-2 expression and controls apoptosis in T-cells in an IL2-dependent manner. Its levels are shown to be upregulated by TGF-1. IKZF3 expression Th17 cells is associated with increased IL-10 production and it is shown to interact with known regulators of IL10 expression. It also acts as a transcriptional activator in CD4+ T cells in cooperation with other factors, such as FOXP3 and BLIMP1 in CD4+ regulatory T cells (Tregs). The degron sequence of IKZF3 is localized to amino acids 144-168 and a Q147H mutation is reported to cripple the function of IKZF3. This sequence constitutes a modular degron that can be used to target heterologous proteins. This degron sequence is as capable as the full-length IKZF3 at targeting selected proteins for degradation. It is shown to target green fluorescent protein (GFP) for pomalidomide-induced degradation when fused to the C-terminus of GFP, but not to the N-terminus. It has also been sown to target exogenous HIF2 for pomalidomide-induced degradation when fused to the C-terminus of HIF2 . Clone 1C3-D3-F8 recognizes the degron sequence that is considered as an important regulator of protein degradation. (Ref.: Koduri, V. et al. (2019). Proc. Natl. Acad. Sci. USA. 116(7); 2539-2544).

Especificidad

Clone 1C3-D3-F8 is a mouse monoclonal antibody that detects IKZF3 Protein. It targets an epitope within 25 amino acids from a Degron sequence in the N-terminal half.

Inmunógeno

KLH-conjugated linear peptide corresponding to Degron sequence from human IKZF3.

Aplicación

Quality Control Testing

Evaluated by Western Blotting in lysate from HEK293 cells expressing IKZF3 Degron-tagged PTPA.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected Zinc finger protein Aiolos (IKZF3) in lysates from HEK293 cells ectopically expressing IKZF3 Degron-tagged PTPA, but not in lysate from untransfected HEK293 cells (wild type).

Anti-IKFZ3 degron tag, clone 1C3-D3-F8, Cat. No. MABE1934, is a mouse monoclonal antibody that detects Zinc finger protein Aiolos (IKZF3) and is tested for use in Western Blotting.

Forma física

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable