MABC86
Anti-Nesprin-2 Antibody, clone K20-478
clone K20-478, from mouse
Sinónimos:
Nesprin-2, Nuclear envelope spectrin repeat protein 2, Nucleus and actin connecting element protein, Protein NUANCE, Synaptic nuclear envelope protein 2, Syne-2
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
K20-478, monoclonal
reactividad de especies
human, mouse
técnicas
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotipo
IgG2bκ
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... SYNE2(23224)
Descripción general
Nesprin-2 (SYNE-2) is also known as Nuclear envelope spectrin repeat protein 2 or Nucleus and actin connecting element protein, and it functions to network organelles and the actin cytoskeleton in order maintain subcellular spatial organization. Nesprin-2 connects nuclei to the cytoskeleton by interacting with the nuclear envelope and cytoplasmic F-actin, and is required for centrosome migration to the apical cell surface during early ciliogenesis. Nesprin-2 is widely expressed, with high levels seen in kidney, adult and fetal liver, stomach and placenta. Defects in SYNE2 are responsible for Emery-Dreifuss muscular dystrophy type 5 (EDMD5).
Inmunógeno
His-tagged recombinant protein corresponding to human Nesprin-2.
Aplicación
Detect Nesprin-2 using this mouse monoclonal antibody, Anti-Nesprin-2 Antibody, clone K20-478 validated for use in western blotting, ICC & IP.
Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected Nesprin-2 in HepG2 and COS-7 cells.
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Nesprin-2 in HA-MKS3 transfected HEK293 whole cell extracts. Immunoprecipitated sample was then subjected to Western Blotting using the same representative lot of antibody (Dawe, H. R., et al. (2009). J Cell Sci. 122(Pt 15): 2716-2626.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Nesperin-2 in IMCDE whole cell extracts. High levels of a 25 kDa ABD-containing isoform was observed only at post-confluence (+72 hours); however, a second major ABD-containing isoform was observed at 55 kDa before and after confluence (Dawe, H. R., et al. (2009). J Cell Sci. 122(Pt 15): 2716-2626.).
Dylight® is a registered trademark of Thermo Fisher Scientific. Alexa Fluor is a registered trademark of Molecular Probes, Inc.
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Nesprin-2 in HA-MKS3 transfected HEK293 whole cell extracts. Immunoprecipitated sample was then subjected to Western Blotting using the same representative lot of antibody (Dawe, H. R., et al. (2009). J Cell Sci. 122(Pt 15): 2716-2626.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Nesperin-2 in IMCDE whole cell extracts. High levels of a 25 kDa ABD-containing isoform was observed only at post-confluence (+72 hours); however, a second major ABD-containing isoform was observed at 55 kDa before and after confluence (Dawe, H. R., et al. (2009). J Cell Sci. 122(Pt 15): 2716-2626.).
Dylight® is a registered trademark of Thermo Fisher Scientific. Alexa Fluor is a registered trademark of Molecular Probes, Inc.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
Calidad
Evaluated by Western Blotting in COS-7 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Nesprin-2 in 10 µg of COS-7 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Nesprin-2 in 10 µg of COS-7 cell lysate.
Descripción de destino
~250 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Forma física
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Almacenamiento y estabilidad
Stable for 1 year at 2-8°C from date of receipt.
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
DyLight is a registered trademark of Pierce Biotechnology, Inc.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Helen R Dawe et al.
Journal of cell science, 122(Pt 15), 2716-2726 (2009-07-15)
Meckel-Gruber syndrome (MKS) is a severe autosomal recessively inherited disorder caused by mutations in genes that encode components of the primary cilium and basal body. Here we show that two MKS proteins, MKS1 and meckelin, that are required for centrosome
Sebastian Kandert et al.
Human molecular genetics, 16(23), 2944-2959 (2007-09-21)
The S143F lamin A/C point mutation causes a phenotype combining features of myopathy and progeria. We demonstrate here that patient dermal fibroblast cells have dysmorphic nuclei containing numerous blebs and lobulations, which progressively accumulate as cells age in culture. The
Yen-Yi Zhen et al.
Journal of cell science, 115(Pt 15), 3207-3222 (2002-07-16)
NUANCE (NUcleus and ActiN Connecting Element) was identified as a novel protein with an alpha-actinin-like actin-binding domain. A human 21.8 kb cDNA of NUANCE spreads over 373 kb on chromosome 14q22.1-q22.3. The cDNA sequence predicts a 796 kDa protein with
Yasunao Kamikawa et al.
Cell death discovery, 7(1), 152-152 (2021-07-07)
The nuclear envelope (NE) safeguards the genome and is pivotal for regulating genome activity as the structural scaffold of higher-order chromatin organization. NE had been thought as the stable during the interphase of cell cycle. However, recent studies have revealed
Maria Chiara Lionetti et al.
Biophysical journal, 118(9), 2319-2332 (2020-04-23)
The nuclear morphology of eukaryotic cells is determined by the interplay between the lamina forming the nuclear skeleton, the chromatin inside the nucleus, and the coupling with the cytoskeleton. Nuclear alterations are often associated with pathological conditions as in Hutchinson-Gilford
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