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Key Documents

MABC538

Sigma-Aldrich

Anti-FANCJ/BACH1 Antibody, clone 2G7

clone 2G7, from mouse

Sinónimos:

Fanconi anemia group J protein, Protein FACJ, ATP-dependent RNA helicase BRIP1, BRCA1-associated C-terminal helicase 1, BRCA1-interacting protein C-terminal helicase 1, BRCA1-interacting protein 1

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

2G7, monoclonal

reactividad de especies

human

técnicas

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotipo

IgG1κ

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... BRIP1(83990)

Descripción general

Bach1 and Bach2 constitute a subfamily of the basic region-leucine zipper family. Bach proteins form heterodimers with small Maf proteins, bind to the Maf recognition element (MARE) and repress target gene expression. Transcription regulator protein BACH2 mediates early B cell differentiation and is specifically expressed in pro and mature B-cells. This protein plays an important role in both the immunoglobulin class switch, oncogene-induced senescence, and somatic hypermutation.

Inmunógeno

Epitope: C-terminus
Linear peptide corresponding to the C-terminus of human FANCJ/BACH1.

Aplicación

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected FANCJ/BACH1 in MCF-7 cells (Cantor, S. B., et al. (2001). Cell. 105(1):149-160.).

Western Blotting Analysis: A representative lot from an independent laboratory detected FANCJ/BACH1 in transfected FA-J cell lysate (Xie, J., et al. (2010). Oncogene. 29(17):2499-2508.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated FANCJ/BACH1 from MCF-7 cell lysate, prior to detecting the immunoprecipitated sample in Western Blot (Cantor, S. B., et al. (2001). Cell. 105(1):149-160.).
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-FANCJ/BACH1 Antibody, clone 2G7 is validated for use in western blotting, ICC & IP for the detection of FANCJ/BACH1.

Calidad

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected FANCJ/BACH1 in 10 µg of MCF-7 cell lysate.

Descripción de destino

~140 kDa observed

Forma física

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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J Xie et al.
Oncogene, 29(17), 2499-2508 (2010-02-23)
BRCA1 and the DNA helicase FANCJ (also known as BACH1 or BRIP1) have common functions in breast cancer suppression and DNA repair. However, the functional significance of the direct interaction between BRCA1 and FANCJ remains unclear. Here, we have discovered
S B Cantor et al.
Cell, 105(1), 149-160 (2001-04-13)
BRCA1 interacts in vivo with a novel protein, BACH1, a member of the DEAH helicase family. BACH1 binds directly to the BRCT repeats of BRCA1. A BACH1 derivative, bearing a mutation in a residue that was essential for catalytic function

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