MABC46
Anti-Atg13 Antibody, clone 2H4.2
clone 2H4.2, from mouse
Sinónimos:
Autophagy-related protein 13
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
2H4.2, monoclonal
reactividad de especies
human
técnicas
immunohistochemistry: suitable
western blot: suitable
isotipo
IgG1κ
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... ATG13(9776)
Descripción general
The autophagy-related protein 13 (Atg13) plays an important role in the formation of autophagosomes. Autophagosomes are formed in response to nutrient deprivation and function as the transport vesicles for organelles, proteins, and protein complexes targeted for lysosomes that digest these cargos to produce energy and nutrients. Atg13 is activated by the mTOR pathway and forms a complex with the FIP200 protein. This complex is involved in enhancing the activity of the ULK1 kinase which is required for the formation of autophagosomes. Atg13/FIP200 faciliates the localization of ULK1 to pre-autophagosomes, and subsequently stabilizes ULK1. Autophagy is an important process in development, growth, and cell differentiation, and disruption of this process may contribute to cancer, aging, and neurodegenerative diseases.
Inmunógeno
GST-tagged recombinant protein corresponding to human Atg13.
Aplicación
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-Atg13 Antibody, clone 2H4.2 is validated for use in Western Blotting, IHC for the detection of Atg13.
Western Blot Analysis: A representative lot detected Atg13 in HeLa cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Atg13 in normal human substancia nigra tissues.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Atg13 in normal human substancia nigra tissues.
Calidad
Evaluated by Western Blot in campthothecin treated HeLa cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Atg13 in 10 µg of campthothecin treated HeLa cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Atg13 in 10 µg of campthothecin treated HeLa cell lysate.
Descripción de destino
~60 kDa observed
Forma física
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Almacenamiento y estabilidad
Stable for 1 year at 2-8°C from date of receipt.
Nota de análisis
Control
Campthothecin treated HeLa cell lysate.
Campthothecin treated HeLa cell lysate.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Yo-Hei Yamamoto et al.
The Journal of cell biology, 219(8) (2020-06-04)
In macroautophagy, membrane structures called autophagosomes engulf substrates and deliver them for lysosomal degradation. Autophagosomes enwrap a variety of targets with diverse sizes, from portions of cytosol to larger organelles. However, the mechanism by which autophagosome size is controlled remains
Marianna Carinci et al.
The EMBO journal, 40(10), e103563-e103563 (2021-05-02)
The early secretory pathway and autophagy are two essential and evolutionarily conserved endomembrane processes that are finely interlinked. Although growing evidence suggests that intracellular trafficking is important for autophagosome biogenesis, the molecular regulatory network involved is still not fully defined.
Suresh Kumar et al.
Developmental cell, 49(1), 130-144 (2019-03-05)
Syntaxin 17 (Stx17) has been implicated in autophagosome-lysosome fusion. Here, we report that Stx17 functions in assembly of protein complexes during autophagy initiation. Stx17 is phosphorylated by TBK1 whereby phospho-Stx17 controls the formation of the ATG13+FIP200+ mammalian pre-autophagosomal structure (mPAS)
Cindy Puente et al.
The Journal of biological chemistry, 291(11), 6026-6035 (2016-01-24)
Autophagy is a conserved catabolic process that utilizes a defined series of membrane trafficking events to generate a de novo double-membrane vesicle termed the autophagosome, which matures by fusing to the lysosome. Subsequently, the lysosome facilitates the degradation and recycling
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