MABC1702

Anti-PDGF-BB Antibody, clone 6A9.9.11

• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 6A9.9.11, monoclonal
• mol peso: calculated mol wt 27.28 kDa; observed mol wt ~12 kDa
• purificado por: using protein G
• reactividad de especies: human
• envase: antibody small pack of 100 μg
• técnicas: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotipo: IgG1κ
• secuencia del epítopo: Internal
• Nº de acceso Protein ID: NP_002599.1
• Nº de acceso UniProt: P01127
• Condiciones de envío: 2-8°C
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

Platelet-derived growth factor subunit B (UniProt: P01127; also known as PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis, Becaplermin) is encoded by the PDGFB (also known as PDGF2, SIS) gene (Gene ID: 5155) in human. PDGFs are a family of four cystine-knot-type growth factors (PDGF-A, -B, -C and -D) that are dimeric mitogenic signaling molecules. In the early developmental stage, they drive the proliferation of undifferentiated mesenchyme and some progenitor populations. During later maturation stages they participate in tissue remodeling, cellular differentiation, and morphogenesis. PDGF A and B can exist as two A subunits (PDGF-AA), two B subunits (PDGF-BB), or one of each (PDGF-AB). These dimeric forms are differentially expressed in various cell types. Their action is mediated via two types of receptors PDGFR and PDGFRβ, which belong to the class III receptor tyrosine kinases and exhibit different expression patterns and physiological roles. PDGFR signaling controls gastrulation and the development of several organs such as lung, intestine, skin, testis, kidney, bones, and neuroprotective tissues and PDGFRβ signaling is an essential regulator of early hematopoiesis and blood vessel formation. PDGF-BB is synthesized with a signal peptide (aa 1-20) and two propeptides (aa 21-81 and 191-241), which are cleaved off to generate the active form (aa 82-190). PDGF-BB is expressed at high levels in the heart, placenta, fetal kidney, and substantia nigra in the brain and at moderate levels in hippocampus, skeletal muscle, kidney, and lung. It is required for normal blood vessel development, and for normal development of kidney glomeruli and is shown to plays an important role in wound healing. It is released by platelets upon wounding. Mutations in PDGFB gene have been linked to idiopathic basal ganglia calcification that leads to a wide spectrum of neuropsychiatric symptoms, including Parkinsonism, dystonia tremor, ataxia, and dementia. (Ref.: Chen, PH., et al. (2013). Biochim. Biophys. Acta. 1834(10); 2176-2186).

Especificidad

Clone 6A9.9.11 is a mouse monoclonal antibody that detects Platelet-derived growth factor subunit B. It targets an epitope within the internal region.

Inmunógeno

Recombinant mature form (aa 82-190) of human Platelet-derived growth factor subunit B (PDGF-BB).

Aplicación

Quality Control Testing

Evaluated by Western Blotting with recombinant human PDGF-BB.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected human recombinant PDGF-BB.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected PDGF-BB in human placenta tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-PDGF-BB, clone 6A9.9.11, Cat. No. MABC1702, is a mouse monoclonal antibody that detects PDGF-BB and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Forma física

Purified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.

Almacenamiento y estabilidad

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable