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Key Documents

MABC1595

Sigma-Aldrich

Anti-RIPK3/RIP3 Antibody, clone 8G7

Sinónimos:

Anti-RIPK3 Antibody, Clone 8G7 Anti-RIPK3, RIPK3 Detection Antibody

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.43

origen biológico

rat

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

8G7, monoclonal

mol peso

calculated mol wt 53.32 kDa
observed mol wt ~53 kDa

reactividad de especies

mouse

envase

antibody small pack of 100 μL

técnicas

immunofluorescence: suitable
western blot: suitable

isotipo

IgG2aκ

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

2-8°C

modificación del objetivo postraduccional

unmodified

Descripción general

Receptor-interacting serine/threonine-protein kinase 3 (UniProt: EC:2.7.11.1, RIP-like protein kinase 3, Receptor-interacting protein 3, RIP-3, mRIP3) is encoded by the Ripk3 (also known as Rip3) gene (Gene ID: 56532) in murine species. RIPK3 is a serine/threonine protein kinase that is expressed in embryo and adult spleen, liver, testis, brain, and lung. Its protein kinase domain is localized in amino acids 22 to 293 in the N-terminal half and it also has a C-terminal RIP homolytic interaction motif (RHIM; aa 440-461), which mediates interaction with the RHIM motif of RIPK1 and facilitate the formation of a necrosome. RIPK3 is shown to activate both necroptosis and apoptosis. Activated RIPK3 forms a necrosis-inducing complex and mediates phosphorylation of MLKL, promoting MLKL localization to the plasma membrane and execution of programmed necrosis that is characterized by calcium influx and plasma membrane damage. Its location is mainly cytoplasmic, however, following infection, it can also be detected in the nucleus. RIPK3 also regulates apoptosis that is dependent on RIPK1, FADD, and CASP8, and is independent of MLKL and RIPK3 kinase activity. It phosphorylates RIPK1 and then RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation. In some cell types, RIPK3 is also reported to restrict viral replication by promoting cell death-independent responses. Clone 8G7 is raised against an extended kinase domain (residues 2-353) of mouse RIPK3 and can detect forms that may not be detected by antibodies directed towards the C-terminus of the full-length RIPK3. (Ref.: Samson, AL., et al. (2021). Cell Death Differ. https://doi.org/10.1038/s41418-021-00742-x; Petrie, EJ., et al. (2019). Cell Rep. 28(13); 3309-3319).

Especificidad

Clone 8G7 is a rat monoclonal antibody that detects murine Receptor-interacting serine/threonine-protein kinase 3 (RIPK3). It targets an epitope within the extended kinase domain that includes most of the N-terminal half and a part of C-terminal half.

Inmunógeno

Recombinant fragment corresponding to 352 amino acids from the extended kinase domain of murine Receptor-interacting serine/threonine-protein kinase 3 (RIPK3).

Aplicación

Quality Control Testing

Evaluated by Western Blotting in Mouse dermal fibroblast lysates.

Western Blotting Analysis: A 1:500 dilution of this antibody detected RIPK3/RIP3 in Mouse dermal fibroblast lysates.

Tested Applications

Western Blotting Analysis: A representative lot detected RIPK3/RIP3 in Western Blotting applications (Petrie, E.J., et. al. (2019). Cell Rep. 28(13):3309-3319).

Immunofluorescence Analysis: A representative lot detected RIPK3/RIP3 in Immunofluorescence applications (Samson, A.L., et. al. (2021). Cell Death Differ. doi: 10.1038/s41418-021-00742-x)

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-RIPK3/RIP3, clone 8G7, Cat. No. MABC1595, is a rat monoclonal antibody that detects RIPK3/RIP3 and is tested for use in Immunofluorescence and Western Blotting.

Forma física

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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