MAB5232

Anti-Presenilin-1 Antibody, loop, a.a. 263-378, CT, clone PS1-loop

ascites fluid, clone PS1-loop, Chemicon^®

• Sinónimos: Anti-ACNINV3, Anti-AD3, Anti-FAD, Anti-PS1, Anti-S182
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• clon: PS1-loop, monoclonal
• application: ICC; IHC; IP; WB
• reactividad de especies: rat, primate, mouse, human
• técnicas: immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• citations: 93

Propiedades

• origen biológico: mouse
• forma del anticuerpo: ascites fluid
• tipo de anticuerpo: primary antibodies
• clon: PS1-loop, monoclonal
• reactividad de especies: rat, primate, mouse, human
• fabricante / nombre comercial: Chemicon^®
• técnicas: immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG1
• Nº de acceso NCBI: NM_000021.2
• Nº de acceso UniProt: P49768
• Condiciones de envío: dry ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PSEN1(5663)

Descripción

Descripción general

Presenilin is a probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Presenilin requires the other members of the gamma-secretase complex to have a protease activity. It may play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. The gamma-secretase complex stimulates cell-cell adhesion through its association with the E-cadherin/catenin complex. Under conditions of apoptosis or calcium influx, it cleaves E-cadherin promoting the disassembly of the E-cadherin/catenin complex and increasing the pool of cytoplasmic beta-catenin, thus negatively regulating Wnt signaling. Futher researcher may implicate the complex in hematopoiesis. Defects in PSEN1 have been implicated in early-onset Alzheimer disease type 3 (AD3) and late-onset Alzheimer disease (LOAD).

Especificidad

Presenilin-1, loop

Inmunógeno

Epitope: C-terminus

Presenilin-1, loop fusion protein (amino acids 263-378), human

Aplicación

Anti-Presenilin-1 Antibody, loop, a.a. 263-378, C-terminus, clone PS1-loop detects level of Presenilin-1 & has been published & validated for use in IC, IH(P), IP & WB.

Immunocytochemistry:
A previous lot of this antibody was used in IC.Acetone or methanol fixed cells; or lightly fixed 2.5-3% PFA (5-10′ RT).

Immunohistochemistry:
A previous lot was used on 3% paraformaldehyde fixed tissue. Works on paraffin embedded tissue sections if fixed with PLP, acetone or other light fixations; 10% formalin fixation is untested.

Immunoprecipitation:
A previous lot of this antibody was used in IP.

Western blot:
Recognizes a protein of approximately 18-20 kD, and occasionally there is a doublet (the top band of which is phosphorylated).

Optimal working dilutions must be determined by the end user

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Calidad

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected PRESENILIN-1 on 10 μg of PC12 lysates.

Descripción de destino

18-20 kDa

Forma física

Mouse monoclonal IgG1 ascites with no preservatives.

Unpurified

Almacenamiento y estabilidad

Stable for 6 months at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análisis

Control
Brain tissue

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable