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Key Documents

MAB5210

Sigma-Aldrich

Anti-Phosphacan Antibody, clone 122.2

ascites fluid, clone 122.2, Chemicon®

Sinónimos:

Receptor Tyrosine Phosphatase beta

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

ascites fluid

tipo de anticuerpo

primary antibodies

clon

122.2, monoclonal

reactividad de especies

rat

fabricante / nombre comercial

Chemicon®

técnicas

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotipo

IgM

Nº de acceso UniProt

Condiciones de envío

dry ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... PTPRZ1(5803)

Descripción general

Phosphacan is a soluble nervous tissue-specific proteoglycan that is thought to regulate axonal outgrowth. It is synthesized by glia and binds to neurons and to the neural cell adhesion molecules tenascin, N-CAM or NG-CAM but not to laminin and fibronectin. Phosphacan acts as a potent inhibitor of cell adhesion and neurite outgrowth.

Especificidad

Phosphacan (Receptor Tyrosine Phosphatase Beta). Recognizes the core protein.

Aplicación

Anti-Phosphacan Antibody, clone 122.2 detects level of Phosphacan & has been published & validated for use in WB, IC, IH.
Research Category
Neuroscience
Research Sub Category
Growth Cones & Axon Guidance
Western blot. Recognizes bands at 250, 190, and 180 kDa on western blots of embryonic rat brain extracts.

Immunocytochemistry: 1:10

Immunohistochemistry on 4% paraformaldehyde fixed tissue: 1:1,000

Immunoprecipitation

Optimal working dilutions must be determined by the end user.

Forma física

Ascites fluid. Liquid. Contains no preservative.

Almacenamiento y estabilidad

Maintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles.

Nota de análisis

Control
POSITIVE CONTROL:

embryonic or early post-natal rat brain.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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To date, no reliable methods have proven effective for treating spinal cord injury (SCI). Even systemic administration of methylprednisolone (MP) remains controversial. We previously reported that intrathecal (i.t.) administration of granulocyte colony-stimulating factor (G-CSF) improves outcome after experimental spinal cord
Bala T S Susarla et al.
Journal of neurochemistry, 119(4), 868-878 (2011-09-08)
Traumatic injury to the CNS results in increased expression and deposition of chondroitin sulfate proteoglycans (CSPGs) that are inhibitory to axonal regeneration. Transforming growth factor-β (TGF-β) has been implicated as a major mediator of these changes, but the mechanisms through
Marc R Del Bigio et al.
Cerebrospinal fluid research, 5, 12-12 (2008-07-12)
The cerebral cortex may be compressed in hydrocephalus and some experiments suggest that movement of extracellular substances through the cortex is impaired. We hypothesized that the extracellular compartment is reduced in size and that the composition of the extracellular compartment
Helen B Treloar et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 29(30), 9405-9416 (2009-07-31)
We recently described the boundary-like expression pattern of the extracellular matrix molecule tenascin-C (Tnc) in the developing mouse olfactory bulb (OB) (Shay et al., 2008). In the present study, we test the hypothesis that Tnc inhibits olfactory sensory neuron (OSN)

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