MAB4381C3
Anti-TRA-1-81 Antibody, clone TRA-1-81, Cy3 conjugate
clone TRA-1-81, from mouse, CY3 conjugate
Sinónimos:
Podocalyxin, GCTM-2 antigen, Gp200, Podocalyxin-like protein 1
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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origen biológico
mouse
Nivel de calidad
conjugado
CY3 conjugate
forma del anticuerpo
saturated ammonium sulfate (SAS) precipitated
tipo de anticuerpo
primary antibodies
clon
TRA-1-81, monoclonal
reactividad de especies
human
técnicas
immunocytochemistry: suitable
isotipo
IgM
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... PODXL(5420)
Descripción general
TRA-1-81 is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). Both TRA-1-81 and TRA-1-60 recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope while TRA-1-81 reacts with an unknown epitope of the same molecule. TRA-1-81 is commonly used as marker of undifferentiated pluripotent human stem cells and is downregulated upon differentiation. TRA-1-81 has recently been identified as podocalyxin.
Especificidad
This antibody recognizes human TRA-1-81.
Inmunógeno
Human embryonal carcinoma cell line 2102Ep
Aplicación
Evaluated by Immunocytochemistry in mouse embryonic stem cells (SCR012). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody did not detect TRA-1-81 in mouse embryonic stem cells (SCR012).
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Pluripotent & Early Differentiation
This Anti-TRA-1-81 Antibody, clone TRA-1-81, Cy3 conjugate is validated for use in ICC for the detection of TRA-1-81.
Calidad
Evaluated by Immunocytochemistry in H9 human embryonic stem cells.
Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected TRA-1-81 in H9 human embryonic stem cells.
Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected TRA-1-81 in H9 human embryonic stem cells.
Descripción de destino
420/250 kDa calculated
Forma física
Purified mouse monoclonal IgM conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.
Almacenamiento y estabilidad
Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
Nota de análisis
Control
H9 human embryonic stem cells
H9 human embryonic stem cells
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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G Badcock et al.
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Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). They express several high molecular weight glycoprotein antigens that are down-regulated upon differentiation. One of these antigens, defined by
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Stem cells (Dayton, Ohio), 28(1), 36-44 (2009-11-06)
The availability of induced pluripotent stem cells (iPSCs) has created extraordinary opportunities for modeling and perhaps treating human disease. However, all reprogramming protocols used to date involve the use of products of animal origin. Here, we set out to develop
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