MAB3738

Anti-PML Antibody, clone 36.1-104

ascites fluid, clone 36.1-104, Chemicon^®

• Sinónimos: Promyelocytic Leukemia Protein, Tripartite Motif Protein 19
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• clon: 36.1-104, monoclonal
• application: ICC; IP; WB
• reactividad de especies: mouse
• técnicas: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• citations: 29

Propiedades

• origen biológico: mouse
• forma del anticuerpo: ascites fluid
• tipo de anticuerpo: primary antibodies
• clon: 36.1-104, monoclonal
• reactividad de especies: mouse
• no debe reaccionar con: human
• fabricante / nombre comercial: Chemicon^®
• técnicas: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG2b
• Nº de acceso NCBI: NM_002675.3; NM_033238.2; NM_033239.2; NM_033240.2; NM_033244.3; NM_033246.2; NM_033247.2; NM_033249.2; NM_033250.2
• Nº de acceso UniProt: P29590
• Condiciones de envío: dry ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PML(5371)

Descripción

Descripción general

PML protein is a tripartite motif (TRIM)-containing nuclear protein that may function as, among other things, a transcription factor, a coactivator of nuclear receptors (Ruggerio, 2000), a regulator of apoptosis (Guo, 2000), a mediator of interferon-induced antiviral response (Regand and Chelbi-Aliz, 2001), and as a suppressor of growth and oncogenic transformation (Mu, 1997). PML is localized to the nucleoplasm and in distinct subnuclear structures referred to as Promyelocytic Leukemia Bodies (also known as Nuclear Domain 10). Localization of PML to Promyelocytic Leukemia Bodies requires modification of PML protein by the Small Ubiquitin Modifier (SUMO) and is required for proper formation and integrity of these subnuclear structures. At least 14 splice variants of PML ranging in molecular weight from 48-97 kDa (predicted) have been described in the literature. The functional significance of the various splice variants is not well understood. In patients with Acute Promyelocytic Leukemia, the PML gene is involved in at least two specific chromosomal translocations that result in the expression of chimeric proteins with the Retinoic Acid Receptor alpha (RARalpha DNA- and Hormone-binding domains; Pandolfi, 2001). All isoforms of PML, as well as the PML-RARalpha chimeric proteins expressed in Type A and Type B APL contain an identical N-terminus but vary in the C-terminal portion of the protein (Jenson, 2001).

Especificidad

Recognizes mouse PML (Promyelocytic Leukemia protein). On western blots of protein extracts from mouse embryo fibroblast (MEF1 cells), MAB3738 recognizes a band migrating at approximately 106 kDa corresponding to PML.

Inmunógeno

His-tagged PML fusion protein corresponding to amino acids 1-581 of mouse PML.

Aplicación

Anti-PML Antibody, clone 36.1-104 is a Mouse Monoclonal Antibody for detection of PML also known as Promyelocytic Leukemia Protein & has been validated in ICC, IP & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Western blot (1:500)

Immunoprecipitation

Immunocytochemistry (1:100)

Optimal working dilutions must be determined by end user.

Descripción de destino

106 kDa

Forma física

Ascites fluid containing no preservatives.

Unpurified

Almacenamiento y estabilidad

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Nota de análisis

Control
POSITIVE CONTROL: Mouse embryo fibroblasts (MEF1 cells).

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable