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MAB371

Sigma-Aldrich

Anti-Cranin Antibody, aa572-604 of human sequence, clone 6C1

ascites fluid, clone 6C1, Chemicon®

Sinónimos:

alpha-dystroglycan

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

ascites fluid

tipo de anticuerpo

primary antibodies

clon

6C1, monoclonal

reactividad de especies

mouse, sheep, rat, human, chicken, Xenopus

fabricante / nombre comercial

Chemicon®

técnicas

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable

isotipo

IgM

Nº de acceso UniProt

Condiciones de envío

dry ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... DAG1(1605)

Especificidad

Cranin (dystroglycan), extracellular site, near the C-terminus of the alpha-subunit. The expression and localization of cranin varies considerably according to the cells physiologic state and differentiaion state. MAB371 recognizes the same peptide region as MAB373 but a different epitope.

Inmunógeno

Epitope: aa572-604 of human sequence
Synthetic peptide corresponding to a.a. 572-604 of human cranin.

Aplicación

Immunohistochemistry on tissue fixed briefly with 0.5% glutaraldehyde, 2% PLP fixation, methanol or acetone. Prolonged fixation must be avoided. Traditional formalin is not recommended. Suggested working dilution 1:1-1:50.

Immunocytochemistry on neuron, astrocyte, smooth muscle, fibroblast and epithelial cell lines.

ELISA

Not suggested for use in Western blot.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure

Cell Structure
Research Sub Category
Cytoskeleton

ECM Proteins
This Anti-Cranin Antibody, aa572-604 of human sequence, clone 6C1 is validated for use in ELISA, IC, IH for the detection of Cranin.

Forma física

Ascites Liquid.

Almacenamiento y estabilidad

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Purificaiton of Cranin, a laminin binding membrane protein
Smalheiser, NR and Kim, E.
The Journal of Biological Chemistry, 270, 15425-15433 (1995)
B J Collins et al.
Endocrinology, 142(7), 3152-3162 (2001-06-21)
Dystroglycan is a high affinity laminin-binding glycoprotein originally described as a member of the dystrophin-associated glycoprotein complex in muscle. We have demonstrated the presence of dystroglycan in the thyroid using immunocytochemistry, immunoblots, ligand binding assays, and relative quantitative RT-PCR. In
X Li et al.
The Journal of cell biology, 153(4), 811-822 (2001-05-16)
Fibroblast growth factors and receptors are intimately connected to the extracellular matrix by their affinity to heparan sulfate proteoglycans. They mediate multiple processes during embryonic development and adult life. In this study, embryonic stem cell-derived embryoid bodies were used to

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