MAB3554

Anti-Cytokeratin 7 Antibody, clone OV-TL 12/30

clone OV-TL 12/30, Chemicon^®, from mouse

• Sinónimos: Anti-Anti-CD20, Anti-Anti-CK-20, Anti-Anti-CK20, Anti-Anti-K20, Anti-Anti-KRT21
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: OV-TL 12/30, monoclonal
• reactividad de especies: human
• fabricante / nombre comercial: Chemicon^®
• técnicas: flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• isotipo: IgG1
• Nº de acceso NCBI: NM_019010.1
• Nº de acceso UniProt: P35900
• Condiciones de envío: dry ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... KRT7(3855)

Descripción

Descripción general

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual cytokeratin polypeptides are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium.Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometry.

Especificidad

Cytokeratin 7. Reacts with specific glandular-type epithelia, and most carcinomas derived there from.

Inmunógeno

Cytoskeletal preparation of OTN-11 ovarian carcinoma cell line.

Aplicación

Anti-Cytokeratin 7 Antibody, clone OV-TL 12/30 detects level of Cytokeratin 7 & has been published & validated for use in FC, WB, IC, IH(P).

Immunoblotting

Immunohistochemistry on frozen tissue sections fixed with methanol. The antibody is also effective on paraffin embedded tissue sections that have been pretreated with 0.1% pronase/0.1% trypsin in PBS, 0.5% pepsin in 0.01N HCl or microwave pretreatment.

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
Cytokeratins

Ligadura / enlace

Replaces: MABT150

Forma física

Format: Purified

Liquid in PBS containing 0.09% sodium azide.

Almacenamiento y estabilidad

Maintain -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable