MAB347
Anti-Growth Associated Protein 43 Antibody, clone 9-1E12
clone 9-1E12, Chemicon®, from mouse
Sinónimos:
Neuromodulin, B-50 Protein, Growth Associated Protein 43, neuromodulin
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
9-1E12, monoclonal
reactividad de especies
feline, rat, hamster
reactividad de especies (predicha por homología)
human
fabricante / nombre comercial
Chemicon®
técnicas
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotipo
IgG1
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... GAP43(2596)
Descripción general
GAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.
Especificidad
GAP-43 (also known as growth associated protein-43, B-50, F1 and pp46), regardless of the protein’s phosphorylation state.
Inmunógeno
GAP-43 purified from rat brain
Aplicación
Anti-Growth Associated Protein 43 Antibody, clone 9-1E12 detects level of Growth Associated Protein 43 & has been published & validated for use in IH, IP & WB.
Immunohistochemistry:
0.1-0.5 µg/mL of a previous lot on 4% paraformaldehyde perfused and fixed rat cerebellum and cerebrum tissue. The addition of 0.01-0.1% Triton X-100 to incubation buffer will increase cellular permeability.
Immunoprecipitation:
A previous lot of this antibody was used in immunoprecipitation.
5-10µg/mL in membrane preparations from 16-18 day embryonic cortical neuronal cultures.
Western Blot Analysis:
MAB347 will detect a single 43-48 kD band in western blots of membrane fractions of growing neurons.
Optimal working dilutions must be determined by the end user.
0.1-0.5 µg/mL of a previous lot on 4% paraformaldehyde perfused and fixed rat cerebellum and cerebrum tissue. The addition of 0.01-0.1% Triton X-100 to incubation buffer will increase cellular permeability.
Immunoprecipitation:
A previous lot of this antibody was used in immunoprecipitation.
5-10µg/mL in membrane preparations from 16-18 day embryonic cortical neuronal cultures.
Western Blot Analysis:
MAB347 will detect a single 43-48 kD band in western blots of membrane fractions of growing neurons.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuroregenerative Medicine
Neuroregenerative Medicine
Calidad
Routinely evaluated by Western Blot on rat brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected GAP-43/B-50 on 10 μg of rat brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected GAP-43/B-50 on 10 μg of rat brain lysates.
Descripción de destino
43-48 kDa
Forma física
Format: Purified
Protein A Purified mouse immunoglobulin IgG1 in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified
Almacenamiento y estabilidad
Maintain at 2-8°C in undiluted for up to 6 months after date of receipt.
Nota de análisis
Control
Rat DRG tissue that has been subjected to a spinal nerve ligation and cultured neurons.
Rat DRG tissue that has been subjected to a spinal nerve ligation and cultured neurons.
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
¿No encuentra el producto adecuado?
Pruebe nuestro Herramienta de selección de productos.
Opcional
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Enhancer of Zeste Homolog 2 Overexpression in Nasopharyngeal Carcinoma: An Independent Poor Prognosticator that Enhances Cell Growth.
Hwang CF, Huang HY, Chen CH, Chien CY, Hsu YC, Li CF, Fang FM
International Journal of Radiation Oncology, Biology, Physics null
Collateral sprouting mechanism after end-to-side nerve repair in the rat.
T Yamauchi, M Maeda, S Tamai, M Tamai, H Yajima, Y Takakura, S Haga, H Yamamoto
Medical Electron Microscopy : Official Journal of the Clinical Electron Microscopy Society of Japan null
Posttranslational membrane attachment and dynamic fatty acylation of a neuronal growth cone protein, GAP-43.
Skene, J H and Virag, I
The Journal of cell biology, 108, 613-624 (1989)
A A Lie et al.
Journal of neuropathology and experimental neurology, 58(5), 451-458 (1999-05-20)
The localization and morphological assessment of plastic or newly formed synapses in the human brain remains difficult due to the lack of specific markers. The ectoenzyme 5'-nucleotidase may represent a useful marker of these structures, since in adult rodents synaptic
Kiyoshi Doi et al.
Neuroreport, 15(6), 945-947 (2004-04-13)
Notch genes encode receptors for signaling pathways that regulate neurogenesis in various tissues. To better understand the roles of Notch genes in olfactory neurogenesis, we studied the expression of Notch family in developing mouse olfactory epithelium. In the earliest stage
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico